EDN1 ELISA Kit (Dog) (OKCA02053)

Catalog No: OKCA02053

Size:96 Wells

Dog EDN1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Dog
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3.5 Hours
• ELISA Kit Linearity:

  Sample Type	Dilution	Mean (%)	Range (%)
  Serum (n=4)	Neat	92	85-96
  Serum (n=4)	1:2	94	90-99
  Serum (n=4)	1:4	89	81-96
  Serum (n=4)	1:8	100	91-108

• ELISA Kit Principle: Aviva Systems Biology EDN1 ELISA Kit (Dog) (OKCA02053) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for EDN1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for EDN1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample EDN1 captured in well.
• ELISA Kit Range: 3.12 pg/mL-200 pg/mL
• ELISA Kit Recovery:

  Sample Type	Mean (%)	Range (%)
  Serum (n=5)	87	82-96
  EDTA plasma (n=4)	97	82-106

• ELISA Kit Reproducibility: Mean Intra-Assay Variation <10% CV (n=20)
  Mean Inter-Assay Variation <12% CV (n=8)
• ELISA Kit Component:

  Component	Amount
  Anti-EDN1 Microplate	96 Wells (12 x 8 Well strips)
  EDN1 Lyophilized Standard	1 x 120 uL
  100X Biotinylated EDN1 Detector Antibody	1 x 120 uL
  100X Avidin-HRP Conjugate	1 x 50 mL
  Sample Diluent	1 x 15 mL
  Detector Antibody Diluent	1 x 15 mL
  Conjugate Diluent	1 x 20 mL
  25X Wash Buffer	1 x 10 mL
  Stop Solution	1 x 10 mL
  TMB Substrate

• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma And Tissue Homogenates
• Sensitivity: 0.78 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: EDN1
• Alias Symbols: PPET1
• NCBI Gene Id: 403424
• Protein Name: Endothelin-1
• Description of Target: Endothelins are endothelium-derived vasoconstrictor peptides.
• Uniprot ID: P13206
• Protein Accession #: NP_001002956
• Nucleotide Accession #: NM_001002956