BCHE ELISA Kit (Dog) (OKEH08710)

Catalog No: OKEH08710

Size:96T

Product Info

• Predicted Species Reactivity: Canis lupus, Dog
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  serum(n=5)	97-109% (n = 20)	106-116% (n = 20)	89-99% (n = 20)	86-98% (n = 20)
  EDTA plasma(n=5)	108-118% (n = 20)	86-96% (n = 20)	87-96% (n = 20)	112-120% (n = 20)
  heparin plasma(n=5)	105-114% (n = 20)	107-117% (n = 20)	106-115% (n = 20)	104-114% (n = 20)

• ELISA Kit Principle: Aviva Systems Biology BCHE ELISA Kit (Dog) (OKEH08710) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCHE has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCHE is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BCHE captured in well.
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 82% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.2% (n = 20)
  Mean Inter-assay CV%: <=7.3% (n = 20)
• ELISA Kit Component:
  • BCHE Microplate: 96 Wells (12 x 8 Well strips)
  • BCHE Lyophilized Standard: 2
  • 100X Biotinylated BCHE Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Esterase with broad substrate specificity. Contributes to the inactivation of the neurotransmitter acetylcholine. Can degrade neurotoxic organophosphate esters.
  Subcellular Location: Secreted
• Reconstitution and Storage: Store as indicated in product manual.
• Specificity: Natural and recombinant Dog Cholinesterase
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: BCHE
• Alias Symbols: Acylcholine acylhydrolase;Butyrylcholine esterase;Choline esterase II;Pseudocholinesterase.
• Protein Name: P32750
• Description of Target: Cholinesterase
• Uniprot ID: https://www.uniprot.org/uniprot/P32750