BCAR3 ELISA Kit (Human) (OKEH08605)

Catalog No: OKEH08605

Size:96T

Human BCAR3 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology BCAR3 ELISA Kit (Human) (OKEH08605) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BCAR3 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BCAR3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BCAR3 captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=5.0% (n = 20)
  Mean Inter-assay CV%: <=7.5% (n = 20)
• ELISA Kit Component:
  • BCAR3 Microplate: 96 Wells (12 x 8 Well strips)
  • BCAR3 Lyophilized Standard: 2
  • 100X Biotinylated BCAR3 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: May act as an adapter protein and couple activated growth factor receptors to a signaling pathway that regulates the proliferation in breast cancer cells. When overexpressed, it confers anti-estrogen resistance in breast cancer cell lines. May also be regulated by cellular adhesion to extracellular matrix proteins.
  Unigene: Hs.36958
  SMR: O75815
  String: 9606.ENSP00000260502
  KEGG: hsa:8412
  MIM: 604704
  Pfam: PF00617
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 22pg/mL
• Specificity: Natural and recombinant Human Breast cancer anti-estrogen resistance protein 3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: BCAR3
• Gene Full Name: BCAR3 adaptor protein, NSP family member
• Alias Symbols: AND-34;BCAR3, NSP family adaptor protein;breast cancer anti-estrogen resistance 3;breast cancer antiestrogen resistance 3 protein;breast cancer anti-estrogen resistance protein 3;dJ1033H22.2 (breast cancer anti-estrogen resistance 3);epididymis secretory sperm binding protein;mig-7;MIG7;migration inducting gene-7;novel SH2-containing protein 2;NSP2;SH2 domain-containing protein 3B;SH2D3B.
• NCBI Gene Id: 8412
• Protein Name: O75815
• Description of Target: Breast cancer anti-estrogen resistance protein 3
• Uniprot ID: https://www.uniprot.org/uniprot/O75815
• Protein Accession #: NP_001248337.1
• Nucleotide Accession #: NM_001261408.1