BCAR1 ELISA Kit (Human) (OKCD01928)

Catalog No: OKCD01928 (Formerly GWB-KBBVF3)

Size:96WELLS

Human BCAR1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	86-101%	82-92%	87-99%	91-102%
  EDTA Plasma (n=5)	86-97%	83-96%	94-103%	81-96%
  Heparin Plasma (n=5)	98-105%	80-99%	87-96%	82-89%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Breast Cancer Anti-Estrogen Resistance 1 (BCAR1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Breast Cancer Anti-Estrogen Resistance 1 (BCAR1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Breast Cancer Anti-Estrogen Resistance 1 (BCAR1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 78.12-5000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	80-99	80
  EDTA Plasma (n=5)	95-103	99
  Heparin Plasma (n=5)	90-98	95

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-BCAR1 Microplate: 96 Wells (12 x 8 Well Strips)
  • BCAR1 Lyophilized Standard: 2 x 5 ng
  • 100X Biotinylated BCAR1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 27 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Breast Cancer Anti-Estrogen Resistance 1 (BCAR1). No significant cross-reactivity or interference between Breast Cancer Anti-Estrogen Resistance 1 (BCAR1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: BCAR1
• Gene Full Name: BCAR1 scaffold protein, Cas family member
• Alias Symbols: BCAR1, Cas family scaffold protein;BCAR1, Cas family scaffolding protein;breast cancer anti-estrogen resistance protein 1;CAS;Cas scaffolding protein family member 1;CAS1;CASS1;CRKAS;CRK-associated substrate;Crk-associated substrate p130Cas;P130Cas.
• NCBI Gene Id: 9564
• Protein Name: Breast cancer anti-estrogen resistance protein 1
• Description of Target: Docking protein which plays a central coordinating role for tyrosine kinase-based signaling related to cell adhesion. Implicated in induction of cell migration. Overexpression confers antiestrogen resistance on breast cancer cells.
• Uniprot ID: P56945
• Protein Accession #: NP_001164185.1
• Nucleotide Accession #: NM_001170714.1
• Protein Size (# AA): 870