BCAM ELISA Kit (Human) (OKCD07619)

Catalog No: OKCD07619

Size:96WELLS

BCAM ELISA Kit (Human) (OKCD07619) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Basal Cell Adhesion Molecule (BCAM). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Basal Cell Adhesion Molecule (BCAM). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Basal Cell Adhesion Molecule (BCAM), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Basal Cell Adhesion Molecule (BCAM) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 1.56-100ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Basal Cell Adhesion Molecule (BCAM) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Basal Cell Adhesion Molecule (BCAM) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • BCAM Microplate: 96 Wells (12 x 8 Well strips)
  • BCAM Lyophilized Standard: 2
  • 100X BCAM HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids.
• Sensitivity: < 0.60ng/mL

Target Info

• Gene Symbol: BCAM
• Gene Full Name: basal cell adhesion molecule (Lutheran blood group)
• Alias Symbols: AU;Auberger b antigen;basal cell adhesion molecule;basal cell adhesion molecule (Lu and Au blood groups);B-CAM cell surface glycoprotein;B-cell adhesion molecule;CD239;F8/G253 antigen;LU;Lutheran antigen;Lutheran blood group glycoprotein;Lutheran blood group variant LUGA;MSK19.
• NCBI Gene Id: 4059
• Protein Name: Basal cell adhesion molecule
• Description of Target: Laminin alpha-5 receptor. May mediate intracellular signaling.
• Uniprot ID: P50895
• Protein Accession #: NP_001013275.1
• Nucleotide Accession #: NM_001013257.2