Bbc3 ELISA Kit (Rat) (OKEH04736)

Catalog No: OKEH04736

Size:96T

Rat Bbc3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Bbc3 ELISA Kit (Rat) (OKEH04736) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Bbc3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Bbc3 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Bbc3 captured in well.
• ELISA Kit Range: 0.156-10ng/ml
• ELISA Kit Reproducibility: Mean Intra-Assay CV: < 10%
  Mean Inter-Assay CV: < 12%
• ELISA Kit Component:
  • Bbc3 Microplate: 96 Wells (12 x 8 Well Strips)
  • Bbc3 Standard: 2
  • Sample Diluent: 1 x 20 mL
  • Bbc3 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other biological fluids.
• Sensitivity: 0.091 ng/mL
• Specificity: Natural and recombinant Rat Bcl-2-binding component 3
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Bbc3
• Gene Full Name: Bcl-2 binding component 3
• Alias Symbols: bcl-2-binding component 3;p53 up-regulated modulator of apoptosis;Puma.
• NCBI Gene Id: 317673
• Protein Name: Q80ZG6
• Description of Target: Bcl-2-binding component 3
• Uniprot ID: https://www.uniprot.org/uniprot/Q80ZG6
• Protein Accession #: NP_776209.1
• Nucleotide Accession #: NM_173837.2
• Protein Size (# AA): 193
• Molecular Weight: 21 kDa