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| Datasheets/Manuals | Printable datasheet for BBC3 Antibody (OAEB00959) |
|---|
| Predicted Species Reactivity | Human |
|---|---|
| Product Format | Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing. |
| Clonality | Polyclonal |
| Host | Goat |
| Application | WB |
| Additional Information | Epitope Info: Internal region Immunogen: Peptide with sequence C-DGGRPQRTVR, from the internal region of the protein sequence according to NP_001120712.1. |
| :: | Reactivity Info: Tested: Human; Expected: Human Product Info: This antibody is expected to recognize isoform 1 (NP_001120712.1) only. |
| Reconstitution and Storage | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20C. Minimize freezing and thawing. |
| Immunogen | BBC3 |
| Purification | Ammonium Sulfate Precipitated; Affinity Purified |
| Predicted Homology Based on Immunogen Sequence | Human: 100% |
| Peptide Sequence | C-DGGRPQRTVR |
| Concentration | 0.5 mg/ml |
| Purity | Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide. |
| Application Info | Peptide ELISA: Antibody detection limit dilution 1:16000. Western Blot: Approx 24kDa band observed in Human peripheral blood mononucleocytes lysates (calculated MW of 26.5kDa according to NP_001120712.1). Recommended concentration: 0.1-0.3ug/ml. |
| Reference | Qiu W, Leibowitz B, Zhang L, Yu J, ; Growth factors protect intestinal stem cells from radiation-induced apoptosis by suppressing PUMA through the PI3K/AKT/p53 axis.; Oncogene 2010 Mar 29 (11): 1622-32.; PMID: 19966853 |
| Gene Symbol | BBC3 |
|---|---|
| Gene Full Name | BCL2 binding component 3 |
| Alias Symbols | bcl-2-binding component 3;JFY1;JFY-1;p53 up-regulated modulator of apoptosis;p53-upregulated modulator of apoptosis;PUMA. |
| NCBI Gene Id | 27113 |
| Protein Name | Bcl-2-binding component 3 |
| Uniprot ID | Q96PG8 |
| Protein Accession # | NP_001120712.1 |
| Molecular Weight | 26.5; 14.5; 10.3; 20.5 |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "BBC3 Antibody (OAEB00959)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human".
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How long will it take to receive "BBC3 Antibody (OAEB00959)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "BBC3 Antibody (OAEB00959)" provided in?
This item is provided in "Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "BBC3 Antibody (OAEB00959)"?
This target may also be called "bcl-2-binding component 3;JFY1;JFY-1;p53 up-regulated modulator of apoptosis;p53-upregulated modulator of apoptosis;PUMA." in publications.
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What is the shipping cost for "BBC3 Antibody (OAEB00959)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "BBC3 Antibody (OAEB00959)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "BBC3 Antibody (OAEB00959)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "26.5; 14.5; 10.3; 20.5".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "BBC3 Antibody (OAEB00959)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "BBC3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "BBC3"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "BBC3"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "BBC3"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "BBC3"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "BBC3"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
