BAX Antibody (OAAB07085)

Catalog No: OAAB07085

Size:400ul

BAX Antibody(Cat. #OAAB07085) western blot analysis in MCF-7 cell line lysates (35ug/lane).This demonstrates the BAX antibody detected the BAX protein (arrow).

BAX is strongly supported by BioGPS gene expression data to be expressed in MCF7

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Monoclonal
• Clone: 557CT5.2.1
• Isotype: IgG2b
• Host: Mouse
• Application: WB
• Additional Information: Function: Accelerates programmed cell death by binding to, and antagonizing the apoptosis repressor BCL2 or its adenovirus homolog E1B 19k protein. Under stress conditions, undergoes a conformation change that causes translocation to the mitochondrion membrane, leading to the release of cytochrome c that then triggers apoptosis. Promotes activation of CASP3, and thereby apoptosis
  Cellular Location: Isoform Alpha: Mitochondrion membrane; Single-pass membrane protein. Cytoplasm. Note=Colocalizes with 14- 3-3 proteins in the cytoplasm. Under stress conditions, undergoes a conformation change that causes release from JNK-phosphorylated 14-3-3 proteins and translocation to the mitochondrion membrane Isoform Gamma: Cytoplasm.
  Tissue Location: Expressed in a wide variety of tissues. Isoform Psi is found in glial tumors. Isoform Alpha is expressed in spleen, breast, ovary, testis, colon and brain, and at low levels in skin and lung. Isoform Sigma is expressed in spleen, breast, ovary, testis, lung, colon, brain and at low levels in skin. Isoform Alpha and isoform Sigma are expressed in pro- myelocytic leukemia, histiocytic lymphoma, Burkitt's lymphoma, T- cell lymphoma, lymphoblastic leukemia, breast adenocarcinoma, ovary adenocarcinoma, prostate carcinoma, prostate adenocarcinoma, lung carcinoma, epidermoid carcinoma, small cell lung carcinoma and colon adenocarcinoma cell lines.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This BAX antibody is generated from mice immunized with a KLH conjugated synthetic peptide between 28-56 amino acids from human BAX .
• Purification: This antibody is purified through a protein G column, followed by dialysis against PBS.
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  BAX is strongly supported by BioGPS gene expression data to be expressed in MCF7

• Application Info: WB: 1:100
• Intended Use: BAX Antibody is for research use only and not for use in diagnostic or therapeutic procedures.

Target Info

• Gene Symbol: BAX
• Alias Symbols: BCL2L4
• NCBI Gene Id: 581
• Protein Name: Apoptosis regulator BAX
• Description of Target: The protein encoded by this gene belongs to the BCL2 protein family. BCL2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. This protein forms a heterodimer with BCL2, and functions as an apoptotic activator. This protein is reported to interact with, and increase the opening of, the mitochondrial voltage-dependent anion channel (VDAC), which leads to the loss in membrane potential and the release of cytochrome c. The expression of this gene is regulated by the tumor suppressor P53 and has been shown to be involved in P53-mediated apoptosis. Multiple alternatively spliced transcript variants, which encode different isoforms, have been reported for this gene.
• Uniprot ID: Q07812
• Protein Accession #: NP_004315.1
• Molecular Weight: 21184 Da