Catalog No: OKCD02007 (Formerly GWB-KBBAF4)
Size:96 Wells
Price: $675.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~3.5 Hours
ELISA Kit PrincipleAviva Systems Biology BASP1 ELISA Kit (Human) (OKCD02007) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BASP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BASP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample BASP1 captured in the well.
ELISA Kit Range0.312 - 20 ng/mL
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level BASP1 was tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level BASP1 was tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
Anti-CILP2 Microplate96 Wells (12 x 8 Well Strips)
CILP2 Lyophilized Standard2 x 20 ng
100X Biotinylated CILP2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.114 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Brain Abundant, Membrane Attached Signal Protein 1 (BASP1). No significant cross-reactivity or interference between Brain Abundant, Membrane Attached Signal Protein 1 (BASP1) and analogues was observed.

A novel urinary biomarker protein panel to identify children with ureteropelvic junction obstruction - A pilot study. J Pediatr Urol. 16, 466.e1-466.e9 (2020). 32620509

Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolBASP1
Gene Full NameBrain abundant membrane attached signal protein 1
Alias SymbolsCAP23, NAP22, CAP-23, NAP-22
NCBI Gene Id10409
Protein NameBrain acid soluble protein 1
Description of TargetThis gene encodes a membrane bound protein with several transient phosphorylation sites and PEST motifs. Conservation of proteins with PEST sequences among different species supports their functional significance. PEST sequences typically occur in proteins with high turnover rates. Immunological characteristics of this protein are species specific. This protein also undergoes N-terminal myristoylation. Alternative splicing results in multiple transcript variants that encode the same protein.
Uniprot IDP80723
Protein Accession #NP_001258535.1
Nucleotide Accession #NM_001271606.1
Protein Size (# AA)227
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