BASP1 ELISA Kit (Human) (OKCD02007)

Catalog No: OKCD02007 (Formerly GWB-KBBAF4)

Size:96 Wells

Human BASP1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3.5 Hours
• ELISA Kit Principle: Aviva Systems Biology BASP1 ELISA Kit (Human) (OKCD02007) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BASP1 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BASP1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample BASP1 captured in the well.
• ELISA Kit Range: 0.312 - 20 ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level BASP1 was tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level BASP1 was tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <10%
  Inter-Assay CV: <12%
• ELISA Kit Component:
  • Anti-CILP2 Microplate: 96 Wells (12 x 8 Well Strips)
  • CILP2 Lyophilized Standard: 2 x 20 ng
  • 100X Biotinylated CILP2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.114 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Brain Abundant, Membrane Attached Signal Protein 1 (BASP1). No significant cross-reactivity or interference between Brain Abundant, Membrane Attached Signal Protein 1 (BASP1) and analogues was observed.
• Publications:

  A novel urinary biomarker protein panel to identify children with ureteropelvic junction obstruction - A pilot study. J Pediatr Urol. 16, 466.e1-466.e9 (2020). 32620509

• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: BASP1
• Gene Full Name: Brain abundant membrane attached signal protein 1
• Alias Symbols: CAP23, NAP22, CAP-23, NAP-22
• NCBI Gene Id: 10409
• Protein Name: Brain acid soluble protein 1
• Description of Target: This gene encodes a membrane bound protein with several transient phosphorylation sites and PEST motifs. Conservation of proteins with PEST sequences among different species supports their functional significance. PEST sequences typically occur in proteins with high turnover rates. Immunological characteristics of this protein are species specific. This protein also undergoes N-terminal myristoylation. Alternative splicing results in multiple transcript variants that encode the same protein.
• Uniprot ID: P80723
• Protein Accession #: NP_001258535.1
• Nucleotide Accession #: NM_001271606.1
• Protein Size (# AA): 227