Basal Ganglia Auto-Antibody (Human) ELISA Kit (OKCA02005)

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Technical Documents

Datasheets/Manuals	Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.

Product Info

Predicted Species Reactivity

Human

ELISA Kit Detection Method

Colorimetric, OD450 nm

ELISA Kit Duration

~ 3.5 Hours

ELISA Kit Principle

Aviva Systems Biology Basal Ganglia Auto-Antibody (Human) ELISA Kit (OKCA02005) is based on standard reverse capture sandwich enzyme-linked immuno-sorbent assay technology. Basal Ganglia antigen has been pre-coated and blocked in a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and washed. An HRP conjugated detector antibody specific for HumanAntibody is added, incubated and followed by washing. An enzymatic reaction is produced through the addition of substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Basal Ganglia Auto-Antibody captured in well.

ELISA Kit Reproducibility

Three different samples of known Basal Ganglia Auto-Antibody concentration were tested across one plate with 20 replicates and across 3 plates, 8 replicates each.
Intra-Assay CV: <15%
Inter-Assay CV: <15%

ELISA Kit Component

Component	Amount
Basal Ganglia Auto-Antibody Microplate	96 Wells (12 x 8 Well strips)
Negative Control	1 x 800 uL
Positive Control	1 x 800 uL
100X Anti-Human Antibody HRP-Conjugate	1 x 120 uL
HRP-Conjugate Diluent	1 x 20 mL
Sample Diluent	2 x 20 mL
25X Wash Buffer	1 x 20 mL
TMB Substrate	1 x 10 mL
Stop Solution	1 x 10 mL