BAP1 Antibody - N-terminal region (OAAB15793)

Catalog No: OAAB15793

Size:400ul

Western blot analysis of BAP1 Antibody (N-term) in mouse testis lysates (35ug/lane). BAP1 (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC-P
• Additional Information: Cellular Location: Cytoplasm. Nucleus. Note=Mainly nuclear. Binds to chromatin. Localizes to the cytoplasm when monoubiquitinated by the E2/E3 hybrid ubiquitin-protein ligase UBE2O (PubMed:24703950).
  Tissue Location: Highly expressed in testis, placenta and ovary. Expressed in breast.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This BAP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 36-66 amino acids of human BAP1.
• Purification: This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Predicted Homology Based on Immunogen Sequence: Bovine, Zebrafish, Rat
• Concentration: 2 mg/ml
• Intended Use: BAP1 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: FC: 1:25
  WB: 1:1000
  WB: 1:2000

Target Info

• Gene Symbol: BAP1
• Alias Symbols: UCHL2, hucep-6, HUCEP-13
• NCBI Gene Id: 8314
• Protein Name: Ubiquitin carboxyl-terminal hydrolase BAP1
• Description of Target: 'BRCA1-associated protein-1,' or BAP1 interacts with the RING finger domain of BRCA1. The N-terminal 240 amino acids of the predicted 729-amino acid human protein show homology to ubiquitin C-terminal hydrolases (UCHs), thiol proteases that catalyze proteolytic processing of ubiquitin. In addition, BAP1 contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals. BAP1 and BRCA1 associate in vivo and have overlapping subnuclear localization patterns. BAP1 enhances BRCA1-mediated inhibition of breast cancer cell growth. Northern blot analysis indicates that BAP1 is expressed as a 4-kb mRNA in all human tissues tested, with A 4.8-kb transcript expressed exclusively in testis. Northern blot analysis and in situ hybridization reveal that BAP1 and BRCA1 are coexpressed during murine breast development and remodeling. The BAP1 gene has been mapped to 3p21.3, a region of loss of heterozygosity for breast cancer as well as frequently deleted in lung carcinomas. Intragenic homozygous rearrangements and deletions of BAP1 appear in lung carcinoma cell lines. It has been postulated that BAP1 is a tumor suppressor gene that functions in the BRCA1 growth control pathway.
• Uniprot ID: Q92560
• Protein Accession #: NP_004647.1
• Molecular Weight: 80 kDa