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Catalog No: OKCD09208
Size:96WELLS
Price: $800.00
SKU
OKCD09208
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Datasheets/ManualsPrintable datasheet for BANP ELISA kit (Human)
Product Info
Predicted Species ReactivityHuman
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColormetric|Regular detection
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to BTG3 Associated Nuclear Protein (BANP). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to BTG3 Associated Nuclear Protein (BANP). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain BTG3 Associated Nuclear Protein (BANP), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of BTG3 Associated Nuclear Protein (BANP) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.156-10ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BTG3 Associated Nuclear Protein (BANP) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BTG3 Associated Nuclear Protein (BANP) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
BANP Microplate96 Wells (12 x 8 Well strips)
BANP Lyophilized Standard2
100X BANP HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and Storage2°C to 8°C, -20°C
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.055ng/mL
Gene SymbolBANP
Alias SymbolsBEND1, SMAR1, SMARBP1
NCBI Gene Id54971
Protein NameProtein BANP
Description of TargetBTG3 associated nuclear protein
Uniprot IDQ8N9N5
Protein Accession #NP_001167010.1
Nucleotide Accession #NM_001173539.1
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