BANF1 Antibody (OAPB00480)

Catalog No: OAPB00480

Size:100UG

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Western blot analysis of BANF1 in human kidney tissue lysate with BANF1 antibody at (A) 0.5 and (B) 1 ug/mL.

Immunofluorescence of BANF1 in human kidney tissue with BANF1 antibody at 20 ug/mL.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid. BANF1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Clonality: Polyclonal
• Host: Rabbit
• Conjugation: Unconjugated
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Western blot
  Buffer: Antibody is supplied in PBS containing 0.02% sodium azide.
• Reconstitution and Storage: BANF1 antibody can be stored at 4C for three months, store at -20C for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Immunogen: Anti-BANF1 antibody (4017) was raised against a peptide corresponding to 15 amino acids near the carboxy terminus of human BANF1. The immunogen is located within the last 50 amino acids of BANF1.
• Purification: Affinity Purified
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: 1 mg/ml
• Blocking Peptide: For anti-BANF1 (OAPB00480) antibody is Catalog # OEPB02597
• Application Info: BANF1 antibody can be used for detection of BANF1 by Western blot at 0.5 - 1 ug/ml. (Optimal dilution should be determined by user) Human kidney tissue lysate can be used as a positive control. This antibody can also be used for immunofluorescence starting at 20 ug/ml. BANF1 antibody is human, mouse and rat reactive.
• Other Applications Image 1 Data: Immunofluorescence of BANF1 in human kidney tissue with BANF1 antibody at 20 ug/mL.
• Reference: Furukawa K. LAP2 binding protein 1 (L2BP1/BAF) is a candidate mediator of LAP2-chromatin interaction. J. Cell Sci. 1999; 112:2485-92.;Cai M, Huang Y, Ghirlando R, et al. Solution structure of the constant region of nuclear envelope protein LAP2 reveals two LEM-domain structures: one binds BAF and the other binds DNA. EMBO J. 2001; 20:4399-407.;Chen H and Engelman A. The barrier-to-autointegration protein is a host factor for HIV type 1 integration. Proc. Natl. Acad. Sci. USA 1998; 95:15270-4.;

Target Info

• Gene Symbol: BANF1
• Gene Full Name: BAF nuclear assembly factor 1
• Alias Symbols: BAF;barrier to autointegration factor 1;barrier-to-autointegration factor;BCRP1;breakpoint cluster region protein 1;D14S1460;NGPS.
• NCBI Gene Id: 8815
• Protein Name: Barrier to autointegration factor 1 [Homo sapiens]|Barrier-to-autointegration factor
• Description of Target: Plays fundamental roles in nuclear assembly, chromatin organization, gene expression and gonad development. May potently compress chromatin structure and be involved in membrane recruitment and chromatin decondensation during nuclear assembly. Contains 2 non-specific dsDNA-binding sites which may promote DNA cross-bridging.
• Uniprot ID: O75531
• Protein Accession #: https://www.ncbi.nlm.nih.gov/protein/AAH05942
• Nucleotide Accession #: NM_001143985.1; NM_003860.3
• Molecular Weight: Predicted: 10kD Observed: 12 kD Despite its predicted molecular weight, BANF1 often migrates at a higher size in SDS-PAGE.