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Catalog No: OPCA04661
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OPCA04661
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BAMA Recombinant Protein (Klebsiella pneumoniae) (OPCA04661)

Product Info
Predicted Species ReactivityKlebsiella pneumoniae
Product FormatLyophilized from 10mM Tris-HCI, 1mM EDTA, 6% Trehalose, pH 8.0.
Additional InformationSpecies Specificity Detail: Klebsiella pneumoniae (strain 342)
Reconstitution and StorageWe recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20°C/-80°C. Our default final concentration of glycerol is 50%. Customers could use it as reference. Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
PurificationAffinity purified using IMAC
ConcentrationVaries by lot. See vial for concentration.
PurityGreater than 90% as determined by SDS-PAGE.
Protein SequencePartial Protein: FVVKDIHFEGLQRVAVGAALLSMPVRPGDTVTDDDISNTIRALFATGNFEDVRVLRDGDTLLVQVKERPTIASITFSGNKSVKDDMLKQNLEASGVRVGESLDRTTIADIEKGLEDFYYSVGKYSASVKAVVTPLPRNRVDLKLVFQEG
SourceE.coli
TagN-terminal 10XHis-B2M-tagged and C-terminal Myc-tagged
ReferenceComplete genome sequence of the N2-fixing broad host range endophyte Klebsiella pneumoniae 342 and virulence predictions verified in mice.Fouts D.E., Tyler H.L., DeBoy R.T., Daugherty S., Ren Q., Badger J.H., Durkin A.S., Huot H., Shrivastava S., Kothari S., Dodson R.J., Mohamoud Y., Khouri H., Roesch L.F.W., Krogfelt K.A., Struve C., Triplett E.W., Methe B.A.PLoS Genet. 4:E1000141-E1000141(2008)
Gene SymbolBAMA
Alias SymbolsbamA, yaeT, KPK_4543Outer membrane protein assembly factor BamA
Protein NameOuter membrane protein assembly factor BamA
Description of TargetPart of the outer membrane protein assembly complex, which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Constitutes, with BamD, the core component of the assembly machinery.
Uniprot IDB5Y1J4
Protein Accession #WP_012542808
Nucleotide Accession #NC_011283
Protein Size (# AA)Recombinant
Molecular Weight34.5kDa
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