Catalog No: OKCD01661 (Formerly GWB-KBBMC2)
Size:96WELLS
Price: $675.00
SKU
OKCD01661
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Datasheets/ManualsPrintable datasheet for BAK1 ELISA Kit (Human) (OKCD01661)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Antagonist/Killer 1 (BAK1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Antagonist/Killer 1 (BAK1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Antagonist/Killer 1 (BAK1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bcl2 Antagonist/Killer 1 (BAK1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range0.625-40ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2 Antagonist/Killer 1 (BAK1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2 Antagonist/Killer 1 (BAK1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
Anti-BAK1 Microplate96 Wells (12 x 8 Well Strips)
BAK1 Lyophilized Standard2 x 40 ng
100X Biotinylated BAK1 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeTissue homogenates, cell lysates and other biological fluids
Sensitivity< 0.239 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Bcl2 Antagonist/Killer 1 (BAK1). No significant cross-reactivity or interference between Bcl2 Antagonist/Killer 1 (BAK1) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolBAK1
Gene Full NameBCL2 antagonist/killer 1
Alias Symbolsapoptosis regulator BAK;BAK;BAK-LIKE;bcl-2 homologous antagonist/killer;bcl2-L-7;BCL2L7;BCL2-like 7 protein;bcl-2-like protein 7;CDN1;pro-apoptotic protein BAK.
NCBI Gene Id578
Protein NameBcl-2 homologous antagonist/killer
Description of TargetPlays a role in the mitochondrial apoptosic process. Upon arrival of cell death signals, promotes mitochondrial outer membrane (MOM) permeabilization by oligomerizing to form pores within the MOM. This releases apoptogenic factors into the cytosol, including cytochrome c, promoting the activation of caspase 9 which in turn processes and activates the effector caspases.
Uniprot IDQ16611
Protein Accession #NP_001179.1
Nucleotide Accession #NM_001188.3
Protein Size (# AA)211
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