BAI3 ELISA Kit (Human) (OKCD08757)

Catalog No: OKCD08757

Size:96WELLS

BAI3 ELISA Kit (Human) (OKCD08757) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Specific Angiogenesis Inhibitor 3 (BAI3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Specific Angiogenesis Inhibitor 3 (BAI3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Specific Angiogenesis Inhibitor 3 (BAI3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Brain Specific Angiogenesis Inhibitor 3 (BAI3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Specific Angiogenesis Inhibitor 3 (BAI3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Specific Angiogenesis Inhibitor 3 (BAI3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • BAI3 Microplate: 96 Wells (12 x 8 Well strips)
  • BAI3 Lyophilized Standard: 2
  • 100X BAI3 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids.
• Sensitivity: < 0.061ng/mL

Target Info

• Gene Symbol: ADGRB3
• Gene Full Name: adhesion G protein-coupled receptor B3
• Alias Symbols: adhesion G protein-coupled receptor B3;BAI3;brain-specific angiogenesis inhibitor 3;dJ91B17.1 (brain-specific angiogenesis inhibitor 3).
• NCBI Gene Id: 577
• Protein Name: Adhesion G protein-coupled receptor B3
• Description of Target: Receptor that plays a role in the regulation of synaptogenesis and dendritic spine formation at least partly via interaction with ELMO1 and RAC1 activity (By similarity). Promotes myoblast fusion through ELMO/DOCK1 (PubMed:24567399).
• Uniprot ID: O60242
• Protein Accession #: NP_001695.1
• Nucleotide Accession #: NM_001704.2