BAI1 ELISA Kit (Human) (OKCD01663)

Catalog No: OKCD01663 (Formerly GWB-KBBDH3)

Size:96WELLS

Human BAI1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	83-105%	78-99%	80-95%	85-99%
  EDTA Plasma (n=5)	79-92%	80-96%	95-102%	87-101%
  Heparin Plasma (n=5)	91-101%	88-102%	84-96%	81-101%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Brain Specific Angiogenesis Inhibitor 1 (BAI1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Brain Specific Angiogenesis Inhibitor 1 (BAI1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Brain Specific Angiogenesis Inhibitor 1 (BAI1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Brain Specific Angiogenesis Inhibitor 1 (BAI1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 31.25-2000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	97-105	101
  EDTA Plasma (n=5)	87-98	92
  Heparin Plasma (n=5)	87-96	92

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Brain Specific Angiogenesis Inhibitor 1 (BAI1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Brain Specific Angiogenesis Inhibitor 1 (BAI1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-BAI1 Microplate: 96 Wells (12 x 8 Well Strips)
  • BAI1 Lyophilized Standard: 2 x 2 ng
  • 100X Biotinylated BAI1 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 13.1 pg/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Brain Specific Angiogenesis Inhibitor 1 (BAI1). No significant cross-reactivity or interference between Brain Specific Angiogenesis Inhibitor 1 (BAI1) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: ADGRB1
• Gene Full Name: adhesion G protein-coupled receptor B1
• Alias Symbols: adhesion G protein-coupled receptor B1;BAI1;brain-specific angiogenesis inhibitor 1;GDAIF.
• NCBI Gene Id: 575
• Protein Name: Adhesion G protein-coupled receptor B1
• Description of Target: Phosphatidylserine receptor which enhances the engulfment of apoptotic cells (PubMed:24509909). Also mediates the binding and engulfment of Gram-negative bacteria (PubMed:26838550). Stimulates production of reactive oxygen species by macrophages in response to Gram-negative bacteria, resulting in enhanced microbicidal macrophage activity (PubMed:26838550). In the gastric mucosa, required for recognition and engulfment of apoptotic gastric epithelial cells (PubMed:24509909). Promotes myoblast fusion (By similarity). Activates the Rho pathway in a G-protein-dependent manner (PubMed:23782696). Inhibits MDM2-mediated ubiquitination and degradation of DLG4/PSD95, promoting DLG4 stability and regulating synaptic plasticity (By similarity). Required for the formation of dendritic spines by ensuring the correct localization of PARD3 and TIAM1 (By similarity). Potent inhibitor of angiogenesis in brain and may play a significant role as a mediator of the p53/TP53 signal in suppression of glioblastoma (PubMed:11875720).
• Uniprot ID: O14514
• Protein Accession #: NP_001693.2
• Nucleotide Accession #: NM_001702.2
• Protein Size (# AA): 1584