BAG6 ELISA Kit (Rat) (OKEH08024)

Catalog No: OKEH08024

Size:96T

Product Info

• Predicted Species Reactivity: Rat, Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology BAG6 ELISA Kit (Rat) (OKEH08024) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for BAG6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BAG6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample BAG6 captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 86% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.9% (n = 20)
  Mean Inter-assay CV%: <=9.8% (n = 20)
• ELISA Kit Component:
  • BAG6 Microplate: 96 Wells (12 x 8 Well strips)
  • BAG6 Lyophilized Standard: 2
  • 100X Biotinylated BAG6 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: May mediate ricin-induced apoptosis.
  Subcellular Location: Cytoplasm Cytosol Nucleus Secreted Exosome Normally localized in cytosol and nucleus, it can also be released extracellularly, in exosomes, by tumor and myeloid dendritic cells.
  Unigene: Rn.203343
  SMR: Q6MG49
  String: 10116.ENSRNOP00000057557
  KEGG: rno:94342
  Pfam: PF12057
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.156ng/mL
• Specificity: Natural and recombinant Rat Large proline-rich protein BAG6
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Bag6
• Gene Full Name: BAG cochaperone 6
• Alias Symbols: Bat3;BCL2-associated athanogene 6;HLA-B-associated transcript 3;large proline-rich protein BAG6;large proline-rich protein BAT3.
• NCBI Gene Id: 94342
• Protein Name: Q6MG49
• Description of Target: Large proline-rich protein BAG6
• Uniprot ID: https://www.uniprot.org/uniprot/Q6MG49
• Protein Accession #: NP_001029140.1
• Nucleotide Accession #: NM_001033968.1