BAG3 ELISA Kit (Human) (OKDD00144)

Catalog No: OKDD00144 (Formerly GWB-KBBHO6)

Size:96 Wells

Human BAG3 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked immunosorbent assay
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~4 Hours
• ELISA Kit Principle: Aviva Systems Biology BAG3 ELISA Kit (Human) (OKDD00144) is based on standard sandwich enzyme-linked immuno-sorbent assay methodology. An antibody for BAG3 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for BAG3 is added, incubated and removed. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample BAG3 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility:

  Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level BAG3 were tested 20 times on one plate, respectively.

  Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level BAG3 were tested on 3 different plates, 8 replicates in each plate.

  Intra-Assay: CV<10%

  Inter-Assay: CV<12%

• ELISA Kit Component:
  • Anti-BAG3 Microplate: 96 Wells (12 x 8 Well Strips)
  • BAG3 Lyophlized Standard: 2 x 10 ng
  • Standard Diluent: 1 x 20 mL
  • 100X BAG3 Biotinylated Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Detection Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Detection Conjugate Diluent: 1 x 12 mL
  • 10X Sample Diluent: 1 x 30 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue homogenates, Cell lysates
• Sensitivity: < 0.069 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of BAG3. No significant cross-reactivity or interference between BAG3 and analogues was observed.
• Assay Info: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: BAG3
• Gene Full Name: BAG cochaperone 3
• Alias Symbols: BAG family molecular chaperone regulator 3;BAG-3;BCL2 associated athanogene 3;Bcl-2-associated athanogene 3;BCL2-binding athanogene 3;bcl-2-binding protein Bis;Bcl-2-binding protein BIS transcript 2;BIS;CAIR-1;docking protein CAIR-1;MFM6.
• NCBI Gene Id: 9531
• Protein Name: BAG family molecular chaperone regulator 3
• Description of Target: Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed:9873016, PubMed:27474739). Has anti-apoptotic activity (PubMed:10597216). Plays a role in the HSF1 nucleocytoplasmic transport (PubMed:26159920).
• Uniprot ID: O95817
• Protein Accession #: NP_004272.2
• Nucleotide Accession #: NM_004281.3
• Protein Size (# AA): 575
• Molecular Weight: 62 kDa