BAG3 ELISA Kit (Human) (OKCD08090)

Catalog No: OKCD08090

Size:96WELLS

BAG3 ELISA Kit (Human) (OKCD08090) Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Bcl2 Associated Athanogene 3 (BAG3). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Bcl2 Associated Athanogene 3 (BAG3). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Bcl2 Associated Athanogene 3 (BAG3), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Bcl2 Associated Athanogene 3 (BAG3) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Bcl2 Associated Athanogene 3 (BAG3) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Bcl2 Associated Athanogene 3 (BAG3) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • BAG3 Microplate: 96 Wells (12 x 8 Well strips)
  • BAG3 Lyophilized Standard: 2
  • 100X BAG3 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.061ng/mL

Target Info

• Gene Symbol: BAG3
• Gene Full Name: BAG cochaperone 3
• Alias Symbols: BAG family molecular chaperone regulator 3;BAG-3;BCL2 associated athanogene 3;Bcl-2-associated athanogene 3;BCL2-binding athanogene 3;bcl-2-binding protein Bis;Bcl-2-binding protein BIS transcript 2;BIS;CAIR-1;docking protein CAIR-1;MFM6.
• NCBI Gene Id: 9531
• Protein Name: BAG family molecular chaperone regulator 3
• Description of Target: Co-chaperone for HSP70 and HSC70 chaperone proteins. Acts as a nucleotide-exchange factor (NEF) promoting the release of ADP from the HSP70 and HSC70 proteins thereby triggering client/substrate protein release. Nucleotide release is mediated via its binding to the nucleotide-binding domain (NBD) of HSPA8/HSC70 where as the substrate release is mediated via its binding to the substrate-binding domain (SBD) of HSPA8/HSC70 (PubMed:9873016, PubMed:27474739). Has anti-apoptotic activity (PubMed:10597216). Plays a role in the HSF1 nucleocytoplasmic transport (PubMed:26159920).
• Uniprot ID: O95817
• Protein Accession #: NP_004272.2
• Nucleotide Accession #: NM_004281.3