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BAG2 Antibody - C-terminal region (ARP42177_T100)

100 ul
$249.00
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Conjugation Options

ARP42177_T100-FITC Conjugated

ARP42177_T100-HRP Conjugated

ARP42177_T100-Biotin Conjugated

Tested Species Reactivity:
Human
Predicted Species Reactivity:
Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish
Product Format:
Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Clonality:
Polyclonal
Host:
Rabbit
Application:
IHC, WB
Reconstitution and Storage:
For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
Gene Symbol:
BAG2
Official Gene Full Name:
BCL2-associated athanogene 2
NCBI Gene Id:
9532
Protein Name:
BAG family molecular chaperone regulator 2
Swissprot Id:
O95816
Protein Accession #:
NP_004273
Nucleotide Accession #:
NM_004282
Alias Symbols:
RP3-496N17.2, BAG-2, KIAA0576, MGC149462, dJ417I1.2
Replacement Item:
This antibody may replace item sc-101216 from Santa Cruz Biotechnology.
Description of Target:
BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.BAG proteins compete with Hip for binding to the Hsc70/Hsp70 ATPase domain and promote substrate release. All the BAG proteins have an approximately 45-amino acid BAG domain near the C terminus but differ markedly in their N-terminal regions. The predicted BAG2 protein contains 211 amino acids. The BAG domains of BAG1, BAG2, and BAG3 interact specifically with the Hsc70 ATPase domain in vitro and in mammalian cells. All 3 proteins bind with high affinity to the ATPase domain of Hsc70 and inhibit its chaperone activity in a Hip-repressible manner.
Protein Size (# AA):
211
Molecular Weight:
23kDa
Purification:
Protein A purified
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express BAG2.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express BAG2.
Immunogen:
The immunogen is a synthetic peptide directed towards the C terminal region of human BAG2
Predicted Homology Based on Immunogen Sequence:
Cow: 100%; Dog: 100%; Guinea Pig: 100%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 100%
Complete computational species homology data:
Anti-BAG2 (ARP42177_T100)
Peptide Sequence:
Synthetic peptide located within the following region: VDQKFQSIVIGCALEDQKKIKRRLETLLRNIENSDKAIKLLEHSKGAGSK
Concentration:
Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
Protein Interactions:
BAG2; UBC; TP53; LGALS3BP; TUBGCP2; TUBG1; STIP1; TAB1; STUB1; DNAJB6; MLF2; TTC1; STXBP2; SGTA; DNAJB1; HSPA8; SHFM1; PINK1; RPA3; RPA2; RPA1; AHNAK; PARK2; LATS2; TARDBP; EGFR; BAG3; HDAC11; SOX2; NPM1; PAN2; WDR83; WDR77; CCAR2; METTL21B; DNAJC6; NOS2;
Blocking Peptide:
For anti-BAG2 (ARP42177_T100) antibody is Catalog # AAP42177 (Previous Catalog # AAPP24602)
Datasheets/Manuals:
Printable datasheet for anti-BAG2 (ARP42177_T100) antibody
Sample Type Confirmation:

BAG2 is supported by BioGPS gene expression data to be expressed in Jurkat

Target Reference:
Arndt,V., (2005) Mol. Biol. Cell 16 (12), 5891-5900
Publications:

Yue, X; Zhao, Y; Liu, J; Zhang, C; Yu, H; Wang, J; Zheng, T; Liu, L; Li, J; Feng, Z; Hu, W; BAG2 promotes tumorigenesis through enhancing mutant p53 protein levels and function. 4, (2015). IHC, WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 26271008

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1 Item(s)

02/01/2017 16:23
  • Overall Experience:
  • Quality:
Product Review: BAG2 antibody - C-terminal region (ARP42177_T100) in siRUVBL transfected human Saos2 cells using Western Blot
Product Page for BAG2 antibody - C-terminal region (ARP42177_T100)

Researcher: Wenwei Hu, Xuetian Yue, Rutgers Cancer Institute of New Jersey.
Application: Western Blotting
Species + Tissue/Cell type:
Lane 1: 20ug untransfected human Saos2 cells
Lane 2: 20ug siRUVBL transfected human Saos2 cells
Primary antibody dilution: 1:1000
Secondary antibody: Anti-rabbit-HRP
Secondary antibody dilution: 1:3000


Questionnaire:
How do Aviva's reagents play a role in your experimental goals? Excellent
How would you rate this antibody on a scale from 1-5 (5=best) nad why? 5
Would you use this antibody in future experiment? Yes
Have you used another antibody which has worked in your application? Not yet.
Do you believe the information about the reagent on Aviva's website is correct? Yes
If the antibody works, do you plan to use it in future experiments or to publish your data? Why or why not? Yes, the band is specific and clear.
How did you store the antibody after re-suspension? Stored at -20C.
Sample Description (please include 1) species type, and 2) cell/tissue type, and 3) how much protein loaded for each lane of your gel): 1)human, 2) human lung cancer cell H1299, 3)20ug
How many different experimental trials were conducted using the antibody sample? 3
How was this sample prepared? Cells were transfected with siRNA either control or siRuvbl2, then lyzed in cell lysis buffer with protease inhibitor.
Primary antibody dilution and incubation time: 1:1000 incubated overnight
Secondary antibody used and dilution and incubation time: Anti-rabbit IgG-HRP, 1:3000, 1h
What controls were used in your experiment (positive/negative)? Untransfected cells, siRUVBL1 cells
Please include your detailed WB Procedure/Protocol here: 20ug of protein was loaded on SDS-Page mini-gel and eletrophorese according to standard protocols. Transfer proteins from the gel to a PVDF membrane. After transfer, block the membrane with 5% non-fat milk for 1h,. Dilute the antibody with blocking solutionat 1:1000 and incubate overnight at 4C. After incubation, wash 3 times with PBST, 5min/wash. Incubate the membrane with 1:3000 anti-rabbit antibody at room temperature for 1h. Wash the membrane 3 times with PBST, 5min/wash. Incubate membrane in ECL solution and then visualized with film.
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