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Datasheets/Manuals | Printable datasheet for BAFF Antibody (OABB02050) |
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Tested Species Reactivity | Human, Mouse, Rat |
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Predicted Species Reactivity | Human|Mouse|Rat |
Product Format | Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Clonality | Polyclonal |
Clone | Polyclonal |
Isotype | Rabbit IgG |
Host | Rabbit |
Application | Western blot |
Additional Information | Notes: Tested Species: In-house tested species with positive results. Other applications have not been tested. Optimal dilutions should be determined by end users. |
:: | Background: BAFF was regularly detected by enzyme-linked immunosorbent assay in brain tissue lysates and in normal spinal fluid, and in astrocytes by double fluorescence microscopy. BAFF was localized in astrocytes close to BAFF-R-expressing immune cells. BAFF receptors were strongly expressed in situ in primary central nervous system (CNS) lymphomas. The TNF superfamily member B cell-activating factor (BAFF) plays an important role in humoral immunity and in autoimmune diseases, including RA.Local BAFF gene targeting inhibited proinflammatory cytokine expression, suppressed generation of plasma cells and Th17 cells, and markedly ameliorated joint pathology. The B cell activating factor BAFF (BlyS/TALL-1/zTNF4) is a tumor necrosis factor (TNF)-related ligand that promotes B cell survival and binds to three receptors (BCMA, TACI, and the recently described BAFF-R). Human BAFF was mapped to chromosome 13q32-34. The standard used in this kit is recombinant soluble human BAFF (A134-L295) with the molecular mass of 19.6KDa. |
Reconstitution and Storage | 2°C to 8°C|-20°C |
Immunogen | E.coli-derived human BAFF recombinant protein (Position: A134-L285). Human BAFF shares 86.1% amino acid (aa) sequence identity with mouse BAFF. |
Purification | Affinity Purified |
Peptide Sequence | Synthetic peptide located within the following region: AVQGPEETVTQDCLQLIADSETPTIQKGSYTFVPWLLSFKRGSALEEKENKILVKETGYFFIYGQVLYTDKTYAMGHLIQRKKVHVFGDELSLVTLFRCIQNMPETLPNNSCYSAGIAKLEEGDELQLAIPRENAQISLDGDVTFFGALKLL |
Concentration | 500 ug/ml |
Specificity | No cross reactivity with other proteins. |
Application Info | Western blot: 0.1-0.5 ug/ml: Human, Mouse, Rat |
Reference | 1. Krumbholz, M., Theil, D., Derfuss, T., Rosenwald, A., Schrader, F., Monoranu, C.-M., Kalled, S. L., Hess, D. M., Serafini, B., Aloisi, F., Wekerle, H., Hohlfeld, R., Meinl, E. BAFF is produced by astrocytes and up-regulated in multiple sclerosis lesions and primary central nervous system lymphoma. J. Exp. Med. 201: 195-200, 2005. 2. Lam, Q. L. K., Ko, O. K. H., Zheng, B.-J., Lu, L. Local BAFF gene silencing suppresses Th17-cell generation and ameliorates autoimmune arthritis. Proc. Nat. Acad. Sci. 105: 14993-14998, 2008. 3. Schiemann, B., Gommerman, J. L., Vora, K., Cachero, T. G., Shulga-Morskaya, S., Dobles, M., Frew, E., Scott, M. L. An essential role for BAFF in the normal development of B cells through a BCMA-independent pathway. Science 293: 2111-2114, 2001. 4. Schneider, P., MacKay, F., Steiner, V., Hofmann, K., Bodmer, J. L., Holler, N., Ambrose, C., Lawton, P., Bixler, S., Acha-Orbea, H., Valmori, D., Romero, P., Werner-Favre, C., Zubler, R. H., Browning, J. L., Tschopp, J. BAFF, a novel ligand of the tumor necrosis factor family, stimulates B cell growth. J. Exp. Med. 189: 1747-1756, 1999. |
Storage Buffer | Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
Description | Rabbit IgG polyclonal antibody for Tumor necrosis factor ligand superfamily member 13B(TNFSF13B) detection. Tested with WB in Human;Mouse;Rat. |
Gene Symbol | TNFSF13B |
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Gene Full Name | TNF superfamily member 13b |
Alias Symbols | ApoL related ligand TALL-1;B lymphocyte stimulator;BAFF;B-cell-activating factor;B-lymphocyte stimulator;BLYS;CD257;delta BAFF;Delta4 BAFF;dendritic cell-derived TNF-like molecule;DTL;epididymis secretory sperm binding protein;TALL1;TALL-1;THANK;TNF and ApoL-related leukocyte expressed ligand 1;TNF- and APOL-related leukocyte expressed ligand 1;TNF homolog that activates apoptosis;TNFSF20;TNLG7A;tumor necrosis factor (ligand) superfamily, member 13b;tumor necrosis factor (ligand) superfamily, member 20;tumor necrosis factor ligand 7A;tumor necrosis factor ligand superfamily member 13B;tumor necrosis factor superfamily member 13b;tumor necrosis factor-like protein ZTNF4;ZTNF4. |
NCBI Gene Id | 10673 |
Protein Name | Tumor necrosis factor ligand superfamily member 13B |
Description of Target | Cytokine that binds to TNFRSF13B/TACI and TNFRSF17/BCMA. TNFSF13/APRIL binds to the same 2 receptors. Together, they form a 2 ligands -2 receptors pathway involved in the stimulation of B- and T-cell function and the regulation of humoral immunity. A third B-cell specific BAFF-receptor (BAFFR/BR3) promotes the survival of mature B-cells and the B-cell response. |
Uniprot ID | Q9Y275 |
Molecular Weight | 31223 MW |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "BAFF Antibody (OABB02050)"?
The tested species reactivity for this item is "Human, Mouse, Rat". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "BAFF Antibody (OABB02050)"?
This item is available "Domestic: within 1-2 week delivery | International: within 1-2 week delivery".
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What buffer format is "BAFF Antibody (OABB02050)" provided in?
This item is provided in "Lyophilized. Each vial contains 5mg BSA, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "BAFF Antibody (OABB02050)"?
This target may also be called "ApoL related ligand TALL-1;B lymphocyte stimulator;BAFF;B-cell-activating factor;B-lymphocyte stimulator;BLYS;CD257;delta BAFF;Delta4 BAFF;dendritic cell-derived TNF-like molecule;DTL;epididymis secretory sperm binding protein;TALL1;TALL-1;THANK;TNF and ApoL-related leukocyte expressed ligand 1;TNF- and APOL-related leukocyte expressed ligand 1;TNF homolog that activates apoptosis;TNFSF20;TNLG7A;tumor necrosis factor (ligand) superfamily, member 13b;tumor necrosis factor (ligand) superfamily, member 20;tumor necrosis factor ligand 7A;tumor necrosis factor ligand superfamily member 13B;tumor necrosis factor superfamily member 13b;tumor necrosis factor-like protein ZTNF4;ZTNF4." in publications.
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What is the shipping cost for "BAFF Antibody (OABB02050)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "BAFF Antibody (OABB02050)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "BAFF Antibody (OABB02050)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "31223 MW".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "BAFF Antibody (OABB02050)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "TNFSF13B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "TNFSF13B"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "TNFSF13B"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "TNFSF13B"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "TNFSF13B"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "TNFSF13B"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.