BAD BH3 Domain Antibody (OAAB09536)

Catalog No: OAAB09536

Size:400ul

Western blot analysis of Bad BH3 Domain antibody (Cat.# OAAB09536) in mouse bladder tissue lysates (35ug/lane). Bad (arrow) was detected using the purified Pab.

Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.

Bad BH3 Domain Antibody (Cat. #OAAB09536) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human, Mouse
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IHC, FC
• Additional Information: Precautions: Bad BH3 Domain Antibody is for research use only and not for use in diagnostic or therapeutic procedures.
  Function: Promotes cell death. Successfully competes for the binding to Bcl-X (L), Bcl-2 and Bcl-W, thereby affecting the level of heterodimerization of these proteins with BAX. Can reverse the death repressor activity of Bcl-X (L), but not that of Bcl-2 (By similarity). Appears to act as a link between growth factor receptor signaling and the apoptotic pathways
  Cellular Location: Mitochondrion outer membrane. Cytoplasm. Note=Upon phosphorylation, locates to the cytoplasm
  Tissue Location: Expressed in a wide variety of tissues.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This Bad BH3 Domain antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 92-127 amino acids from human Bad BH3 Domain.
• Predicted Homology Based on Immunogen Sequence: Human; Mouse
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Application Info: WB: 1:1000
  IHC-P: 1:50~100
  FC: 1:10~50

Target Info

• Gene Symbol: BAD
• Alias Symbols: BBC2, BCL2L8
• NCBI Gene Id: 572
• Protein Name: Bcl2 antagonist of cell death
• Description of Target: Purified Rabbit Polyclonal Antibody (Pab)
• Uniprot ID: Q92934
• Protein Accession #: NP_004313.1
• Molecular Weight: 18392 Da