B7H6 ELISA Kit (Human) (OKEH01750)

Catalog No: OKEH01750

Size:96 Wells

Human B7H6 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• Application: ELISA
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology B7H6 ELISA Kit (Human) (OKEH01750) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for B7H6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for B7H6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample B7H6 captured in well.
• ELISA Kit Range: 0.312 - 20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 1%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: < 5.4% (n = 20)
  Mean Inter-assay CV%: < 6.9% (n = 20)
• ELISA Kit Component:
  • B7H6 Microplate: 96 Wells (12 x 8 Well strips)
  • B7H6 Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated B7H6 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.12 ng/mL
• Specificity: Natural and recombinant Human Natural cytotoxicity triggering receptor 3 ligand 1
• Publications:

  Updated clinical and biological information from the two-stage phase II study of imatinib mesylate in subjects with relapsed/refractory neuroblastoma. Oncoimmunology. 7, e1468953 (2018). 30357053

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: B7H6
• Gene Full Name: natural killer cell cytotoxicity receptor 3 ligand 1
• Alias Symbols: B7H6, B7-H6, DKFZp686O24166
• NCBI Gene Id: 374383
• Protein Name: Natural cytotoxicity triggering receptor 3 ligand 1
• Description of Target: B7H6 belongs to the B7 family (see MIM 605402) and is selectively expressed on tumor cells. Interaction of B7H6 with NKp30 (NCR3, MIM 611550) results in natural killer (NK) cell activation and cytotoxicity (Brandt et al., 2009 [PubMed 19528259]).[supplied by OMIM, Jan 2011]
• Uniprot ID: Q68D85
• Protein Accession #: NP_001189368.1
• Nucleotide Accession #: NM_001202439.2
• Protein Size (# AA): 454
• Molecular Weight: 50 kDa