Catalog No: OKEH01750
Size:96 Wells
Price: $525.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology B7H6 ELISA Kit (Human) (OKEH01750) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for B7H6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for B7H6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample B7H6 captured in well.
ELISA Kit Range0.312 - 20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 1%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 5.4% (n = 20)
Mean Inter-assay CV%: < 6.9% (n = 20)
ELISA Kit Component
B7H6 Microplate96 Wells (12 x 8 Well strips)
B7H6 Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated B7H6 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates , Cell Culture Supernates, Other Biological Fluids
Sensitivity0.12 ng/mL
SpecificityNatural and recombinant Human Natural cytotoxicity triggering receptor 3 ligand 1

Updated clinical and biological information from the two-stage phase II study of imatinib mesylate in subjects with relapsed/refractory neuroblastoma. Oncoimmunology. 7, e1468953 (2018). 30357053

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolB7H6
Gene Full Namenatural killer cell cytotoxicity receptor 3 ligand 1
Alias SymbolsB7H6, B7-H6, DKFZp686O24166
NCBI Gene Id374383
Protein NameNatural cytotoxicity triggering receptor 3 ligand 1
Description of TargetB7H6 belongs to the B7 family (see MIM 605402) and is selectively expressed on tumor cells. Interaction of B7H6 with NKp30 (NCR3, MIM 611550) results in natural killer (NK) cell activation and cytotoxicity (Brandt et al., 2009 [PubMed 19528259]).[supplied by OMIM, Jan 2011]
Uniprot IDQ68D85
Protein Accession #NP_001189368.1
Nucleotide Accession #NM_001202439.2
Protein Size (# AA)454
Molecular Weight50 kDa
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