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B7-1/CD80 ELISA Kit (Mouse) (OKBB00310)

96 Tests
$379.00
In Stock
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Predicted Species Reactivity:
Mouse
Application:
ELISA
Kit Detection Method:
Colorimetric, OD450 nm
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology CD80 ELISA Kit (Mouse) (OKBB00310) is based on standard sandwich enzyme-linked immune-sorbent assay technology. A rat monoclonal antibody specific for CD80 has been pre-coated onto 96-wellplate (12 x 8 Well Strips). Standards (NSO, D37-K245) and test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for CD80 is added, incubated and followed by washing. Avidin-Biotin-Peroxidase Complex is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is visualized through the addition of TMB substrate which is catalyzed by HRP to produce a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample Mouse CD80 captured in well.
Kit Range:
62.5 pg/ml-4,000 pg/ml
Kit Reproducibility:
Intra-Assay PrecisionInter-Assay Precision
Sample123123
Mean (ng/mL)0.281.562.310.251.722.48
St. Dev.0.0150.1110.1130.0160.1380.134
%CV5.357.114.896.48.025.40
Kit Component:
ComponentAmount
Lyophilized recombinant mouse B7-1/CD80  standard10 ng/tube x 2
Anti-mouse B7-1/CD80a  Antibody Well Plate96 Wells
Sample diluent buffer30 mL
Biotinylated anti- mouse B7-1/CD80  antibody130 uL, dilution 1:100
Antibody diluent buffer12 mL
Avidin-Biotin-Peroxidase Complex (ABC)130 uL, dilution 1:100
ABC diluent buffer12 mL
TMB color developing agent10 mL
TMB stop solution10 mL
Additional Information:
Range: 62.5pg/ml-4000pg/ml
:::
Principle: Aviva’s mouse B7-1/CD80 ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. Mouse B7-1/CD80 specific-specific polyclonal antibodies were precoated onto 96-well plates. The mouse specific detection polyclonal antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse B7-1/CD80 amount of sample captured in plate.
:::
Notes: 1. To inspect the validity of experiment operation and the appropriateness of sample dilution proportion, pilot
experiment using standards and a small number of samples is recommended.
2. The TMB Color Developing agent is colorless and transparent before using, contact us freely if it is not the case.
3. Before using the Kit, spin tubes and bring down all components to the bottom of tubes.
4. Duplicate well assay is recommended for both standard and sample testing.
5. Don’t let 96-well plate dry, for dry plate will inactivate active components on plate.
6. Don’t reuse tips and tubes to avoid cross contamination.
7. To avoid to use the reagents from different batches together.
8. In order to avoid marginal effect of plate incubation due to temperature difference ( reaction may be stronger in the
marginal wells), it is suggested that the diluted ABC and TMB solution will be pre-warmed in 37C for 30 min before
using.
Background: The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain. The CD80 and CD86 (601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1,D37—K245,which is composed of two single chains acids with the dipolymer.
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
CD80
Official Gene Full Name:
CD80 antigen
Alias Symbols:
CD80, B7, T-lymphocyte activation antigen CD80
NCBI Gene Id:
12519
Protein Name:
T-lymphocyte activation antigen CD80
Description of Target:
The protein CD80 (Cluster of Differentiation 80) is a molecule found on activated B cells and monocytes which provides a costimulatory signal necessary for T cell activation and survival. It is also known as B7.1. The cDNA for B7-1 predicts a type I membrane protein, i.e., one synthesized with a signal peptide that is cleaved upon translocation across the endoplasmic membrane. The protein is predicted to contain 2 extracellular domains structurally similar to those of Ig, a hydrophobic transmembrane region, and a short cytoplasmic domain[1].The CD80 and CD86 (601020) genes encode B7-1 and B7-2, respectively, which are structurally similar members of the immunoglobulin superfamily expressed on a variety of hematopoietic cell types.[2] stated that B7-1 and B7-2 provide a costimulatory signal to T cells by interacting with CD28 and CTLA4. The standard product used in this kit is recombinant B7-1,D37—K245,which is composed of two single chains acids with the dipolymer.
Swissprot Id:
Q00609
Protein Accession #:
NP_033985.3
Nucleotide Accession #:
NM_009855.2
Sample Type:
cell culture supernates and serum
Sensitivity:
< 10 pg/ml
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express CD80.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express CD80.
Predicted Homology Based on Immunogen Sequence:
No detectable cross-reactivity with any other cytokine.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Target Reference:
[1]. Reeves, R. H., Patch, D., Sharpe, A. H., Borriello, F., Freeman, G. J., Edelhoff, S., Disteche, C. The costimulatory
genes Cd80 and Cd86 are linked on mouse chromosome 16 and human chromosome 3. Mammalian Genome 8:
581-582, 1997.
[2].Selvakumar, A., Mohanraj, B. K., Eddy, R. L., Shows, T. B., White, P. C., Dupont, B. Genomic organization and
chromosomal location of the human gene encoding the B-lymphocyte activation antigen B7. Immunogenetics 36:
175-181, 1992..
Specificity:
There is no detectable cross-reactivity with other relevant proteins.

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