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Catalog No: OKEH08626
Size:96T
Price: $800.00
SKU
OKEH08626
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHomo sapiens, Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Linearity
Sample1:21:41:81:16
serum(n=5)91-91% (n = 20)107-118% (n = 20)106-116% (n = 20)92-101% (n = 20)
EDTA plasma(n=5)105-117% (n = 20)105-115% (n = 20)95-104% (n = 20)94-105% (n = 20)
heparin plasma(n=5)106-116% (n = 20)81-91% (n = 20)110-118% (n = 20)108-120% (n = 20)
ELISA Kit PrincipleAviva Systems Biology B4GALT6 ELISA Kit (Human) (OKEH08626) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for B4GALT6 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for B4GALT6 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample B4GALT6 captured in well.
ELISA Kit Range0.625-40ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 102% (n = 20)
ELISA Kit ReproducibilityMean Intra-assay CV%: <=6.1% (n = 20)
Mean Inter-assay CV%: <=7.8% (n = 20)
ELISA Kit Component
ComponentAmount
B4GALT6 Microplate96 Wells (12 x 8 Well strips)
B4GALT6 Lyophilized Standard2
100X Biotinylated B4GALT6 Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Additional InformationFunction: Catalyzes the synthesis of lactosylceramide (LacCer) via the transfer of galactose from UDP-galactose to glucosylceramide (GlcCer) (PubMed:3099851, PubMed:1551920, PubMed:24498430). LacCer is the starting point in the biosynthesis of all gangliosides (membrane-bound glycosphingolipids) which play pivotal roles in the CNS including neuronal maturation and axonal and myelin formation (By similarity).
::Subcellular Location: Golgi apparatus Golgi stack membrane Single-pass type II membrane protein Trans cisternae of Golgi stack.
::Unigene: Hs.591063
SMR: Q9UBX8
String: 9606.ENSP00000306459
::KEGG: hsa:9331
MIM: 604017
Pfam: PF02709
Reconstitution and StorageStore as indicated in product manual.
Sensitivity0.303ng/mL
SpecificityNatural and recombinant Human Beta-1,4-galactosyltransferase 6
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolB4GALT6
Gene Full Namebeta-1,4-galactosyltransferase 6
Alias SymbolsB4Gal-T6;beta-1,4-galactosyltransferase 6;beta-1,4-GalTase 6;beta4Gal-T6;beta4GalT-VI;glucosylceramide beta-1,4-galactosyltransferase;lacCer synthase;lactosylceramide synthase;UDP-Gal:betaGlcNAc beta 1,4- galactosyltransferase, polypeptide 6;UDP-Gal:beta-GlcNAc beta-1,4-galactosyltransferase 6;UDP-Gal:glucosylceramide beta-1,4-galactosyltransferase;UDP-galactose:beta-N-acetylglucosamine beta-1,4-galactosyltransferase 6.
NCBI Gene Id9331
Protein NameQ9UBX8
Description of TargetBeta-1,4-galactosyltransferase 6
Uniprot IDhttps://www.uniprot.org/uniprot/Q9UBX8
Protein Accession #NP_001317499.1
Nucleotide Accession #NM_001330570.1
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