Catalog No: OKCD01059 (Formerly GWB-KBBIO9)
Size:96 Wells
Price: $675.00
SKU
OKCD01059
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration2.5 Hours
ELISA Kit PrincipleAviva Systems Biology B4GALNT2 ELISA Kit (Human) (OKCD01059) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for B4GALNT2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for B4GALNT2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample B4GALNT2captured in well.
ELISA Kit Range0.312-20 ng/mL
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level B4GALNT2 were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level B4GALNT2 were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <10%
Inter-Assay CV: <12%
ELISA Kit Component
ComponentAmount
Anti-B4GALNT2 Microplate96 Wells (12 x 8 Well Strips)
B4GALNT2 Lyophilized Standard2 x 20 ng
100X Biotinylated B4GALNT2 Detector Antibody120 uL
Avidin/HRP Conjugate120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeTissue homogenates and other biological fluids.
Sensitivity< 0.113 ng/mL
SpecificityThis assay has high sensitivity and excellent specificity for detection of Beta-1,4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2). No significant cross-reactivity or interference between Beta-1,4-N-Acetyl Galactosaminyl Transferase 2 (b4GALNT2) and analogues was observed.
Assay InfoAssay Methodology: Quantitative Sandwich Immunoassay
Gene SymbolB4GALNT2
Gene Full Namebeta-1,4-N-acetyl-galactosaminyltransferase 2
Alias SymbolsB4GALT, GALGT2
NCBI Gene Id124872
Protein Namebeta-1,4 N-acetylgalactosaminyltransferase 2
Description of TargetInvolved in the synthesis of the Sd(a) antigen (Sia-alpha2,3-[GalNAc-beta1,4]Gal-beta1,4-GlcNAc), a carbohydrate determinant expressed on erythrocytes, the colonic mucosa and other tissues. Transfers a beta-1,4-linked GalNAc to the galactose residue of an alpha-2,3-sialylated chain.2 Publications <p>Manually curated information for which there is published experimental evidence.</p> <p><a href="/manual/evidences#ECO:0000269">More…</a></p> Manual assertion based on experiment iniRef.1"Molecular cloning, gene organization and expression of the human UDP-GalNAc:Neu5Acalpha2-3Galbeta-R beta1,4-N-acetylgalactosaminyltransferase responsible for the biosynthesis of the blood group Sda/Cad antigen: evidence for an unusual extended cytoplasmic domain." Montiel M.D., Krzewinski-Recchi M.A., Delannoy P., Harduin-Lepers A. Biochem. J. 373:369-379(2003) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2), FUNCTION, TISSUE SPECIFICITY, VARIANT ASP-40.Ref.2"Molecular cloning of the human beta1,4 N-acetylgalactosaminyltransferase responsible for the biosynthesis of the Sd(a) histo-blood group antigen: the sequence predicts a very long cytoplasmic domain." Lo Presti L., Cabuy E., Chiricolo M., Dall'Olio F. J. Biochem. 134:675-682(2003) [PubMed] [Europe PMC] [Abstract]Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1), FUNCTION, VARIANT ASP-40. <p>Describes the metabolic pathway(s) associated with a protein.</p><p><a href='../manual/pathway' target='_top'>More...</a></p>Pathwayi: protein glycosylationThis protein is involved in the pathway protein glycosylation, which is part of Protein modification. View all proteins of this organism that are known to be involved in the pathway protein glycosylation and in Protein modification.
Swissprot IdQ8NHY0
Protein Accession #NP_001152859
Nucleotide Accession #NM_001159387.1
Protein Size (# AA)566
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express B4GALNT2.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express B4GALNT2.
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