B3GALT5 Antibody - N-terminal region (OAAB07380)

Catalog No: OAAB07380

Size:400ul

B3GALT5 Antibody (N-term) (Cat. #OAAB07380) western blot analysis in NCI-H460 cell line lysates (35ug/lane).This demonstrates the B3GALT5 antibody detected the B3GALT5 protein (arrow).

B3GALT5 is strongly supported by BioGPS gene expression data to be expressed in NCI-H460

B3GALT5 Antibody (N-term) (Cat. #OAAB07380) flow cytometric analysis of NCI-H460 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Product Info

• Predicted Species Reactivity: Human
• Product Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, FC
• Additional Information: Function: Catalyzes the transfer of Gal to GlcNAc-based acceptors with a preference for the core3 O-linked glycan GlcNAc (beta1,3)GalNAc structure. Can use glycolipid LC3Cer as an efficient acceptor.
  Cellular Location: Golgi apparatus membrane; Single-pass type II membrane protein (Potential)
  Tissue Location: Expressed in stomach, jejunum, colon, pancreas, small intestine, testis and gastrointestinal and pancreatic cancer cell lines. Hardly detected in lung, liver, adrenal gland and peripheral blood leukocytes
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This B3GALT5 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 57-84 amino acids from the N-terminal region of human B3GALT5.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Predicted Homology Based on Immunogen Sequence: Human
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Sample Type Confirmation:

  B3GALT5 is strongly supported by BioGPS gene expression data to be expressed in NCI-H460

• Intended Use: B3GALT5 Antibody (N-term) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:1000
  FC: 1:10~50

Target Info

• Gene Symbol: B3GALT5
• Alias Symbols: B3T5, GLCT5, B3GalTx, B3GalT-V, beta3Gal-T5, beta-3-Gx-T5, beta-1,3-GalTase 5
• NCBI Gene Id: 10317
• Protein Name: Beta-1,3-galactosyltransferase 5
• Description of Target: This gene is a member of the beta-1,3-galactosyltransferase (beta3GalT) gene family. This family encodes type II membrane-bound glycoproteins with diverse enzymatic functions using different donor substrates (UDP-galactose and UDP-N-acetylglucosamine) and different acceptor sugars (N-acetylglucosamine, galactose, N-acetylgalactosamine). The beta3GalT genes are distantly related to the Drosophila Brainiac gene and have the protein coding sequence contained in a single exon. The beta3GalT proteins also contain conserved sequences not found in the beta4GalT or alpha3GalT proteins. The carbohydrate chains synthesized by these enzymes are designated as type 1, whereas beta4GalT enzymes synthesize type 2 carbohydrate chains. The ratio of type 1:type 2 chains changes during embryogenesis. By sequence similarity, the beta3GalT genes fall into at least two groups: beta3GalT4 and 4 other beta3GalT genes (beta3GalT1-3, beta3GalT5). This gene encodes the most probable candidate for synthesis of the type 1 Lewis antigens which are frequently found to be elevated in gastrointestinal and pancreatic cancers. The encoded protein is inactive with N-linked glycoproteins and functions in mucin glycosylation. Five transcript variants have been described which differ in the 5' UTR. All transcript variants encode an identical protein.
• Uniprot ID: Q9Y2C3
• Protein Accession #: NP_006048.1
• Molecular Weight: 36189 Da