B3galt2 ELISA Kit (Mouse) (OKCD01852)

Catalog No: OKCD01852 (Formerly GWB-KBBZX6)

Size:96WELLS

Mouse B3galt2 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colormetric|Regular detection
• ELISA Kit Duration: 3h
• ELISA Kit Linearity:

  Sample	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	85-94%	84-92%	99-105%	92-105%
  EDTA Plasma (n=5)	81-105%	89-102%	94-101%	84-95%
  Heparin Plasma (n=5)	94-102%	78-95%	84-92%	88-104%

• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Beta-1,3-Galactosyltransferase 2 (b3GALT2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Beta-1,3-Galactosyltransferase 2 (b3GALT2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Beta-1,3-Galactosyltransferase 2 (b3GALT2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Beta-1,3-Galactosyltransferase 2 (b3GALT2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery:

  Matrix	Recovery Range (%)	Average (%)
  Serum (n=5)	83-97	89
  EDTA Plasma (n=5)	83-103	92
  Heparin Plasma (n=5)	81-95	87

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-1,3-Galactosyltransferase 2 (b3GALT2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-1,3-Galactosyltransferase 2 (b3GALT2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • Anti-B3galt2 Microplate: 96 Wells (12 x 8 Well Strips)
  • B3galt2 Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated B3galt2 Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: 2°C to 8°C, -20°C
• Sample Type: Serum, plasma, tissue homogenates and other biological fluids
• Sensitivity: < 0.073 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Beta-1,3-Galactosyltransferase 2 (b3GALT2). No significant cross-reactivity or interference between Beta-1,3-Galactosyltransferase 2 (b3GALT2) and analogues was observed.
• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: B3GALT2
• Gene Full Name: UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase, polypeptide 2
• Alias Symbols: Beta-1,3-galactosyltransferase 2, Beta-1,3-GalTase 2, Beta3GalT2, Beta3Gal-T2, UDP-Gal:betaGlcNAc beta 1,3-galactosyltransferase-II
• NCBI Gene Id: 26878
• Protein Name: Beta-1,3-galactosyltransferase 2
• Description of Target: Beta-1,3-galactosyltransferase that transfers galactose from UDP-galactose to substrates with a terminal beta-N-acetylglucosamine (beta-GlcNAc) residue. Can also utilize substrates with a terminal galactose residue, albeit with lower efficiency. Involved in the biosynthesis of the carbohydrate moieties of glycolipids and glycoproteins.
• Uniprot ID: O54905
• Protein Accession #: NP_064409.3
• Nucleotide Accession #: NM_020025.4
• Protein Size (# AA): 422