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Catalog No: OKEH00344
Size:96T
Price: $425.00
SKU
OKEH00344
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
COA DatasheetPrintable COA datasheet for OKEH00344
Product Info
Predicted Species ReactivityMouse, Mus musculus
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology B2M ELISA Kit (Mouse) (OKEH00344) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for B2M has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing,a biotinylated detector antibody specific for B2M is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample B2M captured in well.
ELISA Kit Range0.78-50ng/ml
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 95%
ELISA Kit ReproducibilityMean Intra-assay CV%: < 3.9% (n = 20)
Mean Inter-assay CV%: < 7.4% (n = 20)
ELISA Kit Component
ComponentAmount
B2M Microplate96 Wells (12 x 8 Well strips)
B2M Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated B2M Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.41 ng/mL
SpecificityNatural and recombinant Mouse Beta-2-microglobulin
Publications

β2M Signals Monocytes Through Non-Canonical TGFβ Receptor Signal Transduction. Circ Res. (2021). 33508948

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Description
Gene SymbolB2m
Gene Full Namebeta-2 microglobulin
Alias Symbolsbeta2-;beta2m;beta2-m;beta-2-microglobulin;Ly-m1;Ly-m11.
NCBI Gene Id12010
Protein NameP01887
Description of TargetBeta-2-microglobulin
Uniprot IDhttps://www.uniprot.org/uniprot/P01887
Protein Accession #NP_033865.2
Nucleotide Accession #NM_009735.3
Protein Size (# AA)119
Molecular Weight14 kDa
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