B2M Chemi-Luminescent ELISA Kit (Human) (OKCD03315)

Catalog No: OKCD03315

Size:96WELLS

Human B2M ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens|Human
• Application: Chemiluminescent immunoassay
• ELISA Kit Detection Method: Chemiluminescent
• ELISA Kit Duration: 2h, 40min
• ELISA Kit Linearity:

  Sample	1:2	1:4	1:8	1:16
  Serum (n=5)	82-93%	94-102%	82-94%	94-103%
  EDTA Plasma (n=5)	88-96%	83-103%	92-99%	96-103%
  Heparin Plasma (n=5)	96-103%	78-89%	78-94%	93-104%

• ELISA Kit Principle: The microplate provided in this kit has been pre-coated with an antibody specific to Beta-2-Microglobulin (b2M). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Beta-2-Microglobulin (b2M). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Beta-2-Microglobulin (b2M) level in the sample or standard.
• ELISA Kit Range: 68.6-50,000pg/mL
• ELISA Kit Recovery:

  Matrix	Recovery range (%)	Average(%)
  Serum (n=5)	81-102	98
  EDTA Plasma (n=5)	81-105	94
  Heparin Plasma (n=5)	79-89	83

• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Beta-2-Microglobulin (b2M) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Beta-2-Microglobulin (b2M) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • B2M Microplate: 96 Wells (12 x 8 Well strips)
  • B2M Lyophilized Standard: 2
  • 100X B2M Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • 100X Luminol Substrate: 1 x 2 mL
  • Substrate Diluent: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Serum, plasma, urine and other biological fluids
• Sensitivity: 68.6 pg/mL
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: B2M
• Gene Full Name: beta-2-microglobulin
• Alias Symbols: beta chain of MHC class I molecules;beta-2-microglobin;beta-2-microglobulin;IMD43.
• NCBI Gene Id: 567
• Protein Name: Beta-2-microglobulin
• Description of Target: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).
• Uniprot ID: P61769
• Protein Accession #: NP_004039
• Nucleotide Accession #: NM_004048