B2M Antibody (OAEE00028)

Catalog No: OAEE00028

Size:100UG

Surface staining of human peripheral blood cells by mouse monoclonal anti-beta2-microglobulin antibody B2M-02.

Product Info

• Predicted Species Reactivity: Human|Porcine
• Product Format: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
• Clonality: Monoclonal
• Clone: B2M-02
• Isotype: IgG1
• Host: Mouse
• Application: Enzyme-linked immunosorbent assay|Flow cytometry|Immunocytochemistry|Immunohistochemistry-Paraffin|Western blot
• Reconstitution and Storage: 2°C to 8°C
• Immunogen: Purified isolated human beta2-microglobulin.
• Purification: Purified
• Concentration: 1 mg/ml
• Specificity: The antibody B2M-02 reacts with beta2-microglobulin (beta2M) associated with cell-surface MHC Class I molecules and other membrane antigens as well as with soluble beta2-microglobulin. Beta2M is a 12 kDa Ig like glycoprotein expressed on lymphocytes, thymocytes, monocytes, granulocytes, platelets, endothelial cells and epithelial cells. It is absent on erythrocytes.
• Formulation: Purified
• Purity: > 95% (by SDS-PAGE)
• Storage Buffer: Phosphate buffered saline (PBS) with 15 mM sodium azide, approx. pH 7.4
• Reference: Hilgert I, Horejsi V, Kristofova H.: The use of murine monoclonal antibody B2M-01 for detection and purification ofhuman beta 2-microglobulin. Folia Biol (Praha). 1984;30(6):369-76.
  Drbal K, Angelisova P, Hilgert I, Cerny J, Novak P, Horejsi V.: A proteolytically truncated form of free CD18, the common chain of leukocyte integrins, as a novel marker of activated myeloid cells. Blood. 2001 Sep 1;98(5):1561-6.
  

Target Info

• Gene Symbol: B2M
• Gene Full Name: beta-2-microglobulin
• Alias Symbols: beta chain of MHC class I molecules;beta-2-microglobin;beta-2-microglobulin;IMD43.
• NCBI Gene Id: 567
• Protein Name: Beta-2-microglobulin
• Description of Target: Component of the class I major histocompatibility complex (MHC). Involved in the presentation of peptide antigens to the immune system. Exogenously applied M.tuberculosis EsxA or EsxA-EsxB (or EsxA expressed in host) binds B2M and decreases its export to the cell surface (total protein levels do not change), probably leading to defects in class I antigen presentation (PubMed:25356553).
• Uniprot ID: P61769
• Protein Accession #: NP_004039.1
• Nucleotide Accession #: NM_004048.2