AZI2 ELISA Kit (Human) (OKEH07990)

Catalog No: OKEH07990

Size:96T

Human AZI2 ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology AZI2 ELISA Kit (Human) (OKEH07990) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AZI2 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AZI2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AZI2 captured in well.
• ELISA Kit Range: 0.156-10ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 93% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=3.4% (n = 20)
  Mean Inter-assay CV%: <=8.6% (n = 20)
• ELISA Kit Component:
  • AZI2 Microplate: 96 Wells (12 x 8 Well strips)
  • AZI2 Lyophilized Standard: 2
  • 100X Biotinylated AZI2 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Adapter protein which binds TBK1 and IKBKE playing a role in antiviral innate immunity. Activates serine/threonine-protein kinase TBK1 and facilitates its oligomerization. Enhances the phosphorylation of NF-kappa-B p65 subunit RELA by TBK1. Promotes TBK1-induced as well as TNF-alpha or PMA-induced activation of NF-kappa-B. Participates in IFNB promoter activation via TICAM1.
  Subcellular Location: Cytoplasm
  Unigene: Hs.706676
  SMR: Q9H6S1
  String: 9606.ENSP00000419371
  KEGG: hsa:64343
  MIM: 609916
  Pfam: PF12845
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 0.093ng/mL
• Specificity: Natural and recombinant Human 5-azacytidine-induced protein 2
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AZI2
• Gene Full Name: 5-azacytidine induced 2
• Alias Symbols: 5-azacytidine induced gene 2;5-azacytidine-induced protein 2;AZ2;NAK-associated protein 1;NAP1;NF-kappa-B-activating kinase-associated protein 1;TILP.
• NCBI Gene Id: 64343
• Protein Name: Q9H6S1
• Description of Target: 5-azacytidine-induced protein 2
• Uniprot ID: https://www.uniprot.org/uniprot/Q9H6S1
• Protein Accession #: NP_001127904.1
• Nucleotide Accession #: NM_001134432.1