Aviva Systems Biology will be closed in observance of Good Friday 3/29/2024. If you are in need of any assistance please email info@avivasysbio.com

Catalog No: OKCD09291
Size:96WELLS
Price: $750.00
SKU
OKCD09291
Availability: Domestic: within 1-2 weeks delivery | International: within 1-2 weeks delivery
Please contact customer service when ordering 10+ kits.

Bulk
Order
Aviva's
Satisfaction
Guarantee
Contact Us:
Shipping Info:
  • $55: Antibody & Protein in US
  • $55 + $25/Kit in US
  • Contact us for international orders.
Datasheets/ManualsPrintable datasheet for AZGP1 ELISA Kit (Human) (OKCD09291)
Product Info
Predicted Species ReactivityHomo sapiens|Human
ApplicationEnzyme-linked Immunosorbent assay-Sandwich
ELISA Kit Detection MethodColorimetric
ELISA Kit Duration3h
ELISA Kit PrincipleThe test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
ELISA Kit Range4.7-300ng/mL
ELISA Kit ReproducibilityIntra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Alpha-2-Glycoprotein 1, Zinc Binding (aZGP1) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
ELISA Kit Component
ComponentAmount
AZGP1 Microplate96 Wells (12 x 8 Well strips)
AZGP1 Lyophilized Standard2
100X AZGP1 HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 9 mL
Stop Solution1 x 6 mL
Reconstitution and Storage2°C to 8°C|-20°C
Sample TypeSerum, plasma, urine, saliva, seminal plasma, sweat and other biological fluids
Sensitivity< 1.8ng/mL
Gene SymbolAZGP1
Gene Full Namealpha-2-glycoprotein 1, zinc-binding
Alias SymbolsAlpha-2-glycoprotein, zinc;testicular tissue protein Li 227;ZA2G;ZAG;zinc-alpha-2-glycoprotein;zn-alpha-2-glycoprotein;Zn-alpha2-glycoprotein;zn-alpha-2-GP.
NCBI Gene Id563
Protein NameZinc-alpha-2-glycoprotein
Description of TargetStimulates lipid degradation in adipocytes and causes the extensive fat losses associated with some advanced cancers. May bind polyunsaturated fatty acids.
Uniprot IDP25311
Protein Accession #NP_001176.1
Nucleotide Accession #NM_001185.3
Write Your Own Review
You're reviewing:AZGP1 ELISA Kit (Human) (OKCD09291)
Your Rating
We found other products you might like!