AXIN1 ELISA Kit (Mouse) (OKEH04969)

Catalog No: OKEH04969

Size:96 Wells

Mouse AXIN1 ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Mouse
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3 Hours
• ELISA Kit Principle: Aviva Systems Biology Axin1 ELISA Kit (Mouse) (OKEH04969) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Axin1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Axin1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Axin1 captured in the well.
• ELISA Kit Range: 78 - 5000pg/ml
• ELISA Kit Recovery: Mean recovery when spiking into Serum and Plasma = 84.4%
• ELISA Kit Reproducibility: Three samples concentrations were measured in replicate within an assay plate and across replicate assays to assess Intra- and Mean Inter-Assay Precision.
  Mean Intra-Assay Precision - <6.3%, n = 20
  Mean Inter-Assay Precision - <8.2%, n = 20
• ELISA Kit Component:
  • AXIN1 Microplate: 96 Wells (12 x 8 Well strips)
  • AXIN1 Lyophilized Standard: 2
  • 100X Biotinylated AXIN1 Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • Stop Solution: 1 x 10 mL
  • TMB Substrate: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, plasma and other biological fluids
• Sensitivity: 39.8 pg/mL
• Specificity: Natural and recombinant Mouse Axin-1
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AXIN1
• Gene Full Name: Axin 1
• Alias Symbols: Fu, Kb, Ki, Axin, fused, kinky, knobbly, AI316800
• NCBI Gene Id: 12005
• Protein Name: Axin-1
• Description of Target: Component of the beta-catenin destruction complex required for regulating CTNNB1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects; down-regulates CTNNB1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway. In Wnt signaling, probably facilitates the phosphorylation of CTNNB1 and APC by GSK3B. Likely to function as a tumor suppressor. Facilitates the phosphorylation of TP53 by HIPK2 upon ultraviolet irradiation. Enhances TGF-beta signaling by recruiting the RNF111 E3 ubiquitin ligase and promoting the degradation of inhibitory SMAD7. Also component of the AXIN1-HIPK2-TP53 complex which controls cell growth, apoptosis and development.
• Uniprot ID: O35625
• Protein Accession #: NP_033863.2
• Nucleotide Accession #: NM_009733.2
• Protein Size (# AA): 863