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AVPR1A ELISA Kit (Rat) (OKEH03569)

96 Wells
$579.00
In Stock
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Gene Symbol:
AVPR1A
Official Gene Full Name:
arginine vasopressin receptor 1A
NCBI Gene Id:
25107
Alias Symbols:
Antidiuretic hormone receptor 1a, AVPR, AVPR V1a, MGC108538, V1a, V1aR, Vascular/hepatic-type arginine vasopressin receptor, Vasopressin V1a receptor
Description of Target:
Receptor for arginine vasopressin. The activity of this receptor is mediated by G proteins which activate a phosphatidyl-inositol-calcium second messenger system. Involved in social memory formation.
Protein Name:
Vasopressin V1a receptor
Swissprot Id:
P30560
Protein Accession #:
NP_444178.2
Nucleotide Accession #:
NM_053019.2
Predicted Species Reactivity:
Rat
Sample Type:
Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity:
< 0.078 ng/mL
Kit Range:
0.312 - 20 ng/mL
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology AVPR1A ELISA Kit (Rat) (OKEH03569) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Avpr1a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Avpr1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Avpr1a captured in well.
Kit Component:
ComponentAmount
Avpr1a Microplate96 Wells (12 x 8 Well strips)
Avpr1a Lyophilized Standard2
Sample Diluent1 x 20 mL
100X Biotinylated Avpr1a Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Detector Antibody Diluent1 x 10 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Kit Detection Method:
Colorimetric, OD450 nm
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Avpr1a.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Avpr1a.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Reconstitution and Storage:
Store as indicated in product manual.
Specificity:
This assay recognizes recombinant and natural rat V1aR. No significant cross-reactivity or interference was observed.
Assay Info:
Assay Methodology: Quantitative Sandwich Immunoassay
Molecular Weight:
48 kDa
Protein Size (# AA):
424

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