Catalog No: OKCA01026
Size:96 Wells
Price: $750.00
SKU
OKCA01026
Availability: Domestic: within 1-2 weeks delivery | International: 1-2 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivitySheep
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3.5 Hours
ELISA Kit Linearity
DilutionMean n=4 (%)Range (%)
Neat9186-94
1:210195-105
1:49287-95
1:89894-102
ELISA Kit PrincipleAviva Systems Biology AVP ELISA Kit (Sheep) (OKCA01026) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. A antibody specific for AVP has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AVP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm and is quantitatively proportional to the amount of sample AVP captured in well.
ELISA Kit Range1.56 pg/mL-100 pg/mL
ELISA Kit Recovery
MatrixRecovery range (%)Average(%)
Serum (n=5)9589-98
EDTA Plasma (n=4)9286-97
ELISA Kit ReproducibilityIntra-assay Precision: 3 samples with known low, middle and high level AVP were tested with 20 replicates across one plate.
Inter-assay Precision: 3 samples with known low, middle and high level AVP were tested on three different plates with 8 replicates, respectively.
Intra-Assay CV: <8%
Inter-Assay CV: <10%
ELISA Kit Component
ComponentAmount
AVP Microplate96 Wells (12 x 8 Well strips)
AVP Lyophilized Standard2
100X Biotinylated AVP Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 50 mL
Detector Antibody Diluent1 x 15 mL
Conjugate Diluent1 x 15 mL
25X Wash Buffer1 x 20 mL
Stop Solution1 x 10 mL
TMB Substrate1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates
Sensitivity0.39 pg/mL
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Protocol Information
Gene SymbolAVP
Gene Full NameVasopressin-neurophysin 2-copeptin
Protein NameVasopressin-neurophysin 2-copeptin
Description of TargetNeurophysin 2 specifically binds vasopressin. Vasopressin has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels.
Uniprot IDP01181
Protein Accession #NP_001119813.1
Nucleotide Accession #NM_001126341.1
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