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AVP ELISA Kit (Rat) (OKEH03099)

96 Wells
$529.00
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Predicted Species Reactivity:
Rat
Kit Detection Method:
Colorimetric, OD450 nm
Kit Duration:
~ 3 Hours
Kit Principle:
Aviva Systems Biology AVP ELISA Kit (Rat) (OKEH03099) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat Avp antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Avp and incubated. The Avp found in the sample or standards competes with the biotinylated Avp for limited binding sites on the immobilized anti-rat Avp antibody. Excess unbound biotinylated Avp and sample or standard Avp is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Avp captured in the well and inversely proportional to the amount of Avp which was contained in the sample or standard.
Kit Range:
31.2 - 2000pg/mL
Kit Component:
ComponentAmount
Anti-Avp Microplate96 Wells (12 x 8 Well strips)
Avp Lyophilized Standard2
100X Biotinylated Avp Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and Storage:
Store as indicated in product manual.
Gene Symbol:
AVP
Official Gene Full Name:
arginine vasopressin
Alias Symbols:
AVP-NPII, DI, Vas, Vasopressin-neurophysin 2-copeptin
NCBI Gene Id:
24221
Protein Name:
Vasopressin-neurophysin 2-copeptin
Description of Target:
Neurophysin 2 specifically binds vasopressin. Vasopressin has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels.
Swissprot Id:
P01186
Protein Accession #:
NP_058688.2
Nucleotide Accession #:
NM_016992.2
Sample Type:
Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity:
15.78 pg/mL
Tissue Tool:
Find tissues and cell lines supported by DNA array analysis to express Avp.
RNA Seq:
Find tissues and cell lines supported by RNA-seq analysis to express Avp.
Datasheets/Manuals:
Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Specificity:
Natural and recombinant Rat Vasopressin-neurophysin 2-copeptin
Assay Info:
Assay Methodology: Quantitative Sandwich ELISA
Molecular Weight:
18 kDa
Protein Size (# AA):
168

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