Catalog No: OKEH03099
Size:96 Wells
Price: $600.00
SKU
OKEH03099
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AVP ELISA Kit (Rat) (OKEH03099) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat Avp antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Avp and incubated. The Avp found in the sample or standards competes with the biotinylated Avp for limited binding sites on the immobilized anti-rat Avp antibody. Excess unbound biotinylated Avp and sample or standard Avp is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Avp captured in the well and inversely proportional to the amount of Avp which was contained in the sample or standard.
ELISA Kit Range31.2 - 2000pg/mL
ELISA Kit Component
ComponentAmount
Anti-Avp Microplate96 Wells (12 x 8 Well strips)
Avp Lyophilized Standard2
100X Biotinylated Avp Detector Antibody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Sample Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids
Sensitivity15.78 pg/mL
SpecificityNatural and recombinant Rat Vasopressin-neurophysin 2-copeptin
Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolAVP
Gene Full Namearginine vasopressin
Alias SymbolsDI, VP, ADH, Vas
NCBI Gene Id24221
Protein NameVasopressin-neurophysin 2-copeptin
Description of TargetNeurophysin 2 specifically binds vasopressin. Vasopressin has a direct antidiuretic action on the kidney, it also causes vasoconstriction of the peripheral vessels.
Swissprot IdP01186
Protein Accession #NP_058688.2
Nucleotide Accession #NM_016992.2
Protein Size (# AA)168
Molecular Weight18 kDa
Tissue ToolFind tissues and cell lines supported by DNA array analysis to express Avp.
RNA SeqFind tissues and cell lines supported by RNA-seq analysis to express Avp.
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