AVP ELISA Kit (Rat) (OKEH03099)

Be the first to review this product

Rat AVP ELISA kit standard curve

Technical Documents

Datasheets/Manuals	Click here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.

Product Info

Predicted Species Reactivity

Rat, Rattus norvegicus

Application

Enzyme-linked Immunosorbent assay-Sandwich

ELISA Kit Detection Method

Colorimetric, OD450 nm

ELISA Kit Duration

~ 3 Hours

ELISA Kit Principle

Aviva Systems Biology AVP ELISA Kit (Rat) (OKEH03099) is based on a competitive enzyme immuno assay technique. The microtiter well-plate in this kit has been pre-coated with an anti-rat Avp antibody. Sample or standards are added to the wells along with a fixed quantity of biotinylated Avp and incubated. The Avp found in the sample or standards competes with the biotinylated Avp for limited binding sites on the immobilized anti-rat Avp antibody. Excess unbound biotinylated Avp and sample or standard Avp is washed from the plate. Avidin-HRP conjugate is added, incubated and washed. An enzymatic reaction is then produced through the addition of TMB substrate which is catalyzed by the immobilized HRP to generate a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration is measured by reading the absorbance at 450 nm which is quantitatively proportional to the amount of biotinylated Avp captured in the well and inversely proportional to the amount of Avp which was contained in the sample or standard.

ELISA Kit Range

31.2-2000pg/mL

ELISA Kit Component

Component	Amount
Anti-Avp Microplate	96 Wells (12 x 8 Well strips)
Avp Lyophilized Standard	2
100X Biotinylated Avp Detector Antibody	1 x 120 uL
100X Avidin-HRP Conjugate	1 x 120 uL
Sample Diluent	1 x 20 mL
Detector Antibody Diluent	1 x 12 mL
Conjugate Diluent	1 x 12 mL
25X Wash Buffer	1 x 30 mL
TMB Substrate	1 x 10 mL
Stop Solution	1 x 10 mL

Reconstitution and Storage

Store as indicated in product manual.

Sample Type

Serum, Plasma, Tissue homogenates, Cell culture supernates, Other biological fluids

Sensitivity

15.78 pg/mL

Specificity

Natural and recombinant Rat Vasopressin-neurophysin 2-copeptin

Assay Info

Assay Methodology: Quantitative Sandwich ELISA

Target Info

Gene Symbol	Avp
Gene Full Name	arginine vasopressin
Alias Symbols	ADH;antidiuretic hormone;arginine vasopressin (Diabetes insipidus), same as Di (conflicting physical mapping);AVP-NPII;DI;prepropressophysin;preprovasopressin-neurophysin;Vas;vasopressin;vasopressin-neurophysin 2-copeptin;vasopressin-neurophysin prepropeptide;VP.
NCBI Gene Id	24221
Protein Name	P01186
Description of Target	Vasopressin-neurophysin 2-copeptin
Uniprot ID	https://www.uniprot.org/uniprot/P01186
Protein Accession #	NP_058688.2
Nucleotide Accession #	NM_016992.2
Protein Size (# AA)	168
Molecular Weight	18 kDa