Catalog No: OKCD08532
Size:96 Wells
Price: $850.00
SKU
OKCD08532
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityRat
ApplicationELISA
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology AVP ELISA Kit (Rat) (OKCD08532) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AVP has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AVP is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AVP captured in the well.
ELISA Kit Range15.6-1000pg/mL
ELISA Kit ReproducibilityIntra-assay reproducibility was evaluated using 20 replicates of 3 samples representing low, middle and high level AVP. Inter-assay reproducibility was evaluated with 3 samples representing low, middle and high level AVP using 8 replicates on each of 3 plates.
Intra-Assay CV <10%
Inter-Assay CV <12%
ELISA Kit Component
ComponentAmount
AVP Microplate96 Wells (12 x 8 Well strips)
AVP Lyophilized Standard2
100X AVP HRP-Detector Anitbody1 x 120 uL
100X Avidin-HRP Conjugate1 x 120 uL
Standard Diluent1 x 20 mL
Detector Antibody Diluent1 x 12 mL
Conjugate Diluent1 x 12 mL
30X Wash Buffer1 x 20 mL
TMB Substrate1 x 2 mL
Stop Solution1 x 20 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Sensitivity< 6.1pg/mL
Gene SymbolAVP
Gene Full Namearginine vasopressin
Alias SymbolsDI, VP, ADH, Vas
NCBI Gene Id24221
Protein Namevasopressin-neurophysin 2-copeptin
Description of Targetneuropeptide hormone; involved in vasoconstriction to regulate blood pressure and smooth muscle contraction during parturition and lactation.
Swissprot IdP01186
Protein Accession #NP_058688.2
Nucleotide Accession #NM_016992.2
Tissue ToolFind tissues and cell lines supported by RNA-seq analysis to express AVP.
RNA SeqFind tissues and cell lines supported by DNA array analysis to express AVP.
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