AVIL ELISA Kit (Human) (OKEH07841)

Catalog No: OKEH07841

Size:96T

Human AVIL ELISA Standard Curve

Product Info

• Predicted Species Reactivity: Homo sapiens, Human
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Principle: Aviva Systems Biology AVIL ELISA Kit (Human) (OKEH07841) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AVIL has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AVIL is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample AVIL captured in well.
• ELISA Kit Range: 78-5000pg/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 101% (n = 20)
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <=2.9% (n = 20)
  Mean Inter-assay CV%: <=8.7% (n = 20)
• ELISA Kit Component:
  • AVIL Microplate: 96 Wells (12 x 8 Well strips)
  • AVIL Lyophilized Standard: 2
  • 100X Biotinylated AVIL Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Sample Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Additional Information: Function: Ca(2+)-regulated actin-binding protein. May have a unique function in the morphogenesis of neuronal cells which form ganglia. Required for SREC1-mediated regulation of neurite-like outgrowth. Plays a role in regenerative sensory axon outgrowth and remodeling processes after peripheral injury in neonates. Involved in the formation of long fine actin-containing filopodia-like structures in fibroblast. Plays a role in ciliogenesis.
  Subcellular Location: Cytoplasm Cytoskeleton Cell projection Cell projection Axon
  Unigene: Hs.584854
  SMR: O75366
  String: 9606.ENSP00000257861
  KEGG: hsa:10677
  MIM: 613397
  Pfam: PF00626
• Reconstitution and Storage: Store as indicated in product manual.
• Sensitivity: 23pg/mL
• Specificity: Natural and recombinant Human Advillin
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: AVIL
• Gene Full Name: advillin
• Alias Symbols: ADVIL;advillin;DOC6;NPHS21;p92.
• NCBI Gene Id: 10677
• Protein Name: O75366
• Description of Target: Advillin
• Uniprot ID: https://www.uniprot.org/uniprot/O75366
• Protein Accession #: NP_006567.3
• Nucleotide Accession #: NM_006576.3