AURKB ELISA Kit (Human) (OKCD01854)

Catalog No: OKCD01854 (Formerly GWB-KBBGV7)

Size:96 Wells

Human AURKB ELISA Kit Standard Curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~3.5 Hours
• ELISA Kit Principle: Aviva Systems Biology AURKB ELISA Kit (Human) (OKCD01854) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for AURKB has been pre-coated onto a 96-wellplate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for AURKB is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution.The density of yellow coloration is read by absorbance at 450 nm and is quantitatively proportional to the amount of sample AURKB captured in the well.
• ELISA Kit Range: 0.156 - 10 ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision: 3 samples with known low, middle and high level AURKB was tested with 20 replicates across one plate.
  Inter-assay Precision: 3 samples with known low, middle and high level AURKB was tested on three different plates with 8 replicates, respectively.
  Intra-Assay CV: <10%
  Inter-Assay CV: <12%
• ELISA Kit Component:
  • Anti-AURKB Microplate: 96 Wells (12 x 8 Well Strips)
  • AURKB Lyophilized Standard: 2 x 10 ng
  • 100X Biotinylated AURKB Detector Antibody: 120 uL
  • Avidin/HRP Conjugate: 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 9 mL
  • Stop Solution: 1 x 6 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Tissue homogenates, cell lysates and other biological fluids
• Sensitivity: < 0.056 ng/mL
• Specificity: This assay has high sensitivity and excellent specificity for detection of Aurora Kinase B (AURKB). No significant cross-reactivity or interference between Aurora Kinase B (AURKB) and analogues was observed.
• Publications:

  Dual Kit/Aur Inhibitors as Chemosensitizing Agents for the Treatment of Melanoma: Design, Synthesis, Docking Studies and Functional Investigation. Sci Rep. 9, 9943 (2019). 31289333

• Assay Info: Assay Methodology: Quantitative Sandwich Immunoassay

Target Info

• Gene Symbol: AURKB
• Gene Full Name: Aurora kinase B
• Alias Symbols: AIK2, AIM1, ARK2, AurB, IPL1, STK5, AIM-1, ARK-2, STK-1, STK12, PPP1R48, aurkb-sv1, aurkb-sv2
• NCBI Gene Id: 9212
• Protein Name: Aurora kinase B
• Description of Target: Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis.
• Uniprot ID: Q96GD4
• Protein Accession #: NP_001243763.1
• Nucleotide Accession #: NM_001256834.2
• Protein Size (# AA): 344