AURKB Antibody (OAPA00363)

Catalog No: OAPA00363 (Formerly GWB-BSP819)

Size:5UG

Product Info

• Predicted Species Reactivity: Human
• Product Format: 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide.
• Clonality: Monoclonal
• Clone: PAT2B1AT
• Isotype: Mouse IgG2b heavy chain and kappa light chain.
• Host: Mouse
• Application: IF
  Product Introduction: Aurora Kinase B (AURKB) belongs to a family of mitotic serine/threonine kinases. AURKB connects with chromosomes for the period of prophase prior to relocalizing to the spindle at anaphase. AURKB localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. AURKB controls chromosome segregation through the control of microtubule-kinetochore attachment and cytokinesis. AURKB is required for kinetochore localization of BUB1 and SGOL1. AURKB expression during the G2/M phase transition is firmly coordinated with histone H3 phosphorylation, while overexpression is seen in many kinds of cancers. AURKB phosphorylates ''Ser-10'' and ''Ser-28'' of histone H3 during mitosis. AURKB is a component of the CPC (chromosomal passenger complex), which is a complex that acts as a key regulator of mitosis. High level expression of AURKB is seen in the thymus, which is also expressed in the spleen, lung, testis, colon, placenta and fetal liver. AURKB is express
• Reconstitution and Storage: For periods up to 1 month store at 4C, for longer periods of time, store at -20C. Prevent freeze thaw cycles.
  Stability: 12 months at -20C. 1 month at 4C.
• Immunogen: The immunogen for anti-AURKB antibody: anti-human AURKB mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with recombinant human AURKB amino acids 1-344 purified from E. coli.
• Predicted Homology Based on Immunogen Sequence: Human
• Purity: AURKB antibody was purified from mouse ascitic fluids by protein-G affinity chromatography.
• Application Info: AURKB antibody has been tested by ELISA and Immunofluorescence analyses to assure specificity and reactivity. Since applications vary, each investigation should be titrated by a reagent to obtain optimal results. Recommended dilution range for Immunofluorescence is 1:1000 ~ 2000. Recommended starting dilution is 1:1000.

Target Info

• Gene Symbol: AURKB
• Gene Full Name: Aurora kinase B
• Alias Symbols: AIK2, AIM1, ARK2, AurB, IPL1, STK5, AIM-1, ARK-2, STK-1, STK12, PPP1R48, aurkb-sv1, aurkb-sv2
• NCBI Gene Id: 9212
• Protein Name: Aurora kinase B
• Description of Target: Mouse Anti Human Aurora Kinase B
• Uniprot ID: Q96GD4
• Protein Accession #: NP_001243763.1; NP_001271455.1; NP_004208.2
• Nucleotide Accession #: NM_001256834.1; NM_001284526.1; NM_004217.3