AURB/C Antibody (Phospho-Thr236/202) (OAAF00261)

Catalog No: OAAF00261 (Formerly GWB-ASB539)

Size:100 ug

Immunohistochemistry analysis of paraffin-embedded human brain, using AurB/C (Phospho-Thr236/202) Antibody. The picture on the right is treated with the synthesized peptide.

Product Info

• Predicted Species Reactivity: Human|Mouse|Rat
• Product Format: Liquid
• Clonality: Polyclonal
• Host: Rabbit
• Application: Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry-Paraffin
• Reconstitution and Storage: -20°C
• Immunogen: The antiserum was produced against synthesized peptide derived from human AurB/C around the phosphorylation site of Thr236/202.
• Purification: The antibody was purified from rabbit antiserum by affinity-chromatography using phospho-peptide. The antibody against non-phosphopeptide was removed by chromatography using corresponding non-phospho peptide.
• Specificity: AurB/C (Phospho-Thr236/202) antibody detects endogenous levels of AurB/C only when phosphorylated at threonine 236/202.
• Formulation: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Dilution: The optimal working dilution should be determined by the end user.
• Application Info: IHC: 1:50~1:100; ELISA: 1:10000
• Reference: Steven L. Warner. Mol. Cancer Ther., Oct 2006; 5: 2450 - 2458.
  Adrianne E. Hontz. AACR Meeting Abstracts, Apr 2005; 2005: 492.
  Adrianne E. Hontz. AACR Meeting Abstracts, Apr 2006; 2006: 1326.

Target Info

• Gene Symbol: AURKB|AURKC
• Gene Full Name: aurora kinase B|aurora kinase C
• Alias Symbols: AIE2;AIK2;AIK3;AIM1;AIM-1;ARK2;ARK-2;ARK3;ARK-3;AurB;AurC;aurkb-sv1;aurkb-sv2;Aurora 1;aurora 3;aurora- and Ipl1-like midbody-associated protein 1;aurora kinase B;aurora kinase B-Sv1;aurora kinase B-Sv2;aurora kinase C;aurora/IPL1/EG2 protein 2;aurora/IPL1-related kinase 2;aurora/IPL1-related kinase 3;aurora-1;aurora-B;aurora-C;aurora-related kinase 2;aurora-related kinase 3;epididymis secretory protein Li 90;HEL-S-90;IPL1;PPP1R48;protein phosphatase 1, regulatory subunit 48;serine/threonine kinase 12;serine/threonine kinase 13 (aurora/IPL1-like);serine/threonine-protein kinase 12;serine/threonine-protein kinase 13;serine/threonine-protein kinase 5;serine/threonine-protein kinase aurora-B;serine/threonine-protein kinase aurora-C;SPGF5;STK-1;STK12;STK13;STK5.
• NCBI Gene Id: 6795|9212
• Protein Name: Aurora kinase B|Aurora kinase C
• Description of Target: Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Involved in the bipolar attachment of spindle microtubules to kinetochores and is a key regulator for the onset of cytokinesis during mitosis. Required for central/midzone spindle assembly and cleavage furrow formation. Key component of the cytokinesis checkpoint, a process required to delay abscission to prevent both premature resolution of intercellular chromosome bridges and accumulation of DNA damage: phosphorylates CHMP4C, leading to retain abscission-competent VPS4 (VPS4A and/or VPS4B) at the midbody ring until abscission checkpoint signaling is terminated at late cytokinesis (PubMed:22422861, PubMed:24814515). AURKB phosphorylates the CPC complex subunits BIRC5/survivin, CDCA8/borealin and INCENP. Phosphorylation of INCENP leads to increased AURKB activity. Other known AURKB substrates involved in centromeric functions and mitosis are CENPA, DES/desmin, GPAF, KIF2C, NSUN2, RACGAP1, SEPT1, VIM/vimentin, HASPIN, and histone H3. A positive feedback loop involving HASPIN and AURKB contributes to localization of CPC to centromeres. Phosphorylation of VIM controls vimentin filament segregation in cytokinetic process, whereas histone H3 is phosphorylated at 'Ser-10' and 'Ser-28' during mitosis (H3S10ph and H3S28ph, respectively). A positive feedback between HASPIN and AURKB contributes to CPC localization. AURKB is also required for kinetochore localization of BUB1 and SGO1. Phosphorylation of p53/TP53 negatively regulates its transcriptional activity. Key regulator of active promoters in resting B- and T-lymphocytes: acts by mediating phosphorylation of H3S28ph at active promoters in resting B-cells, inhibiting RNF2/RING1B-mediated ubiquitination of histone H2A and enhancing binding and activity of the USP16 deubiquitinase at transcribed genes.|Serine/threonine-protein kinase component of the chromosomal passenger complex (CPC), a complex that acts as a key regulator of mitosis. The CPC complex has essential functions at the centromere in ensuring correct chromosome alignment and segregation and is required for chromatin-induced microtubule stabilization and spindle assembly. Plays also a role in meiosis and more particularly in spermatogenesis. Has redundant cellular functions with AURKB and can rescue an AURKB knockdown. Like AURKB, AURKC phosphorylates histone H3 at 'Ser-10' and 'Ser-28'. AURKC phosphorylates the CPC complex subunits BIRC5/survivin and INCENP leading to increased AURKC activity. Phosphorylates TACC1, another protein involved in cell division, at 'Ser-228'.
• Uniprot ID: Q96GD4|Q9UQB9
• Protein Accession #: NP_001015878.1
• Molecular Weight: 39 kDa