ATXN1 ELISA Kit (Mouse) (OKWB00241)

Catalog No: OKWB00241

Size:96T

Mouse ATXN1 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Mouse|Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD 450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Linearity:

  Sample Type	1:2 Dilution	1:4 Dilution	1:8 Dilution	1:16 Dilution
  Serum (n=5)	91-99%	85-104%	91-102%	85-105%
  EDTA Plasma (n=5)	85-90%	85-101%	83-100%	93-100%
  Heparin Plasma (n=5)	83-100%	80-98%	86-98%	82-99%

• ELISA Kit Principle: Aviva Systems Biology Atxn1 ELISA Kit (Mouse) (OKWB00241) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Atxn1 has been pre-coated onto a 96-well plate (12 x 8 Well Strips). Standards or test samples are added to the wells, incubated and removed. A biotinylated detector antibody specific for Atxn1 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes to yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Atxn1 captured in the well.
• ELISA Kit Range: 15.625-1000pg/ml
• ELISA Kit Recovery:

  Sample Type	Range	Mean
  Serum (n=5)	91-105	98
  EDTA Plasma (n=5)	86-94	90
  Heparin Plasma (n=5)	86-100	92

• ELISA Kit Reproducibility: Mean Intra-Assay Precision <10%
  Mean Inter-Assay Precision <12%
• ELISA Kit Component:
  • Atxn1 Microplate: 96 Wells (12 x 8 Well strips)
  • Atxn1 Lyophilized Standard: 2 x 1 ng
  • 100X Atxn1 Biotinylated Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Cell Cultures, Tissue Homogenates, Other Biological Fluids
• Sensitivity: 9.4 pg/mL
• Assay Info: Assay Type: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Atxn1
• Gene Full Name: ataxin 1
• Alias Symbols: 2900016G23Rik;ataxin-1;Atx;Atx1;C85907;Gm10786;Sc;Sca1;spinocerebellar ataxia 1 homolog;Spinocerebellar ataxia type 1 protein homolog.
• NCBI Gene Id: 20238
• Protein Name: Ataxin-1
• Description of Target: Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression (By similarity). May be involved in RNA metabolism (By similarity). In concert with CIC and ATXN1L, involved in brain development (PubMed:28288114).
• Uniprot ID: P54254
• Protein Accession #: NP_001186233
• Nucleotide Accession #: NM_001199304