ATXN1 Antibody (OAAB01518)

Catalog No: OAAB01518

Size:400ul

Anti-ATXN1 Antibody (S776) at 1:2000 dilution + HT-29 whole cell lysate Lysates/proteins at 20 ug per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size :87kDa Blocking/Dilution buffer: 5% NFDM/TBST.

Confocal immunofluorescent analysis of ATXN1 Antibody (OAAB01518) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).

Product Info

• Predicted Species Reactivity: Human
• Product Format: Liquid. PBS with 0.09% (W/V) sodium azide.
• Clonality: Polyclonal
• Isotype: Ig
• Host: Rabbit
• Application: WB, IF
• Additional Information: Function: Chromatin-binding factor that repress Notch signaling in the absence of Notch intracellular domain by acting as a CBF1 corepressor. Binds to the HEY promoter and might assist, along with NCOR2, RBPJ-mediated repression. Binds RNA in vitro. May be involved in RNA metabolism.
  Cellular Location: Cytoplasm. Nucleus Note=Colocalizes with USP7 in the nucleus
  Tissue Location: Widely expressed throughout the body.
• Reconstitution and Storage: Maintain refrigerated at 2-8C for up to 2 weeks. For long term storage store at -20C in small aliquots to prevent freeze-thaw cycles.
• Immunogen: This ATXN1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 754-781 amino acids from human ATXN1.
• Purification: This antibody is purified through a protein A column, followed by peptide affinity purification.
• Concentration: Approximately 0.5mg/ml. Actual concentration varies with each lot.
• Intended Use: ATXN1 Antibody (S776) is for research use only and not for use in diagnostic or therapeutic procedures.
• Application Info: WB: 1:2000
  IF: 1:10~50

Target Info

• Gene Symbol: ATXN1
• Alias Symbols: ATX1, SCA1, D6S504E
• NCBI Gene Id: 6310
• Protein Name: Ataxin-1
• Description of Target: The autosomal dominant cerebellar ataxias (ADCA) are a heterogeneous group of neurodegenerative disorders characterized by progressive degeneration of the cerebellum, brain stem and spinal cord. Clinically, ADCA has been divided into three groups: ADCA types I-III. ADCAI is genetically heterogeneous, with five genetic loci, designated spinocerebellar ataxia (SCA) 1, 2, 3, 4 and 6, being assigned to five different chromosomes. ADCAII, which always presents with retinal degeneration (SCA7), and ADCAIII often referred to as the `pure' cerebellar syndrome (SCA5), are most likely homogeneous disorders. Several SCA genes have been cloned and shown to contain CAG repeats in their coding regions. ADCA is caused by the expansion of the CAG repeats, producing an elongated polyglutamine tract in the corresponding protein. The expanded repeats are variable in size and unstable, usually increasing in size when transmitted to successive generations. The function of the ataxins is not known.
• Uniprot ID: P54253
• Protein Accession #: NP_000323.2
• Molecular Weight: 87 kDa