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- $55: Antibody & Protein in US
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| Datasheets/Manuals | Printable datasheet for ATR Antibody (Phospho-Ser428) (OAAF00519) |
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| Predicted Species Reactivity | Human|Mouse|Rat |
|---|---|
| Product Format | Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl. |
| Clonality | Polyclonal |
| Isotype | IgG |
| Host | Rabbit |
| Application | Enzyme-linked immunosorbent assay|Immunofluorescence|Immunohistochemistry|Immunohistochemistry-Paraffin |
| Additional Information | Modification Sites: H:S428 |
| Reconstitution and Storage | -20°C |
| Immunogen | The antiserum was produced against synthesized peptide derived from human ATR around the phosphorylation site of Ser428. |
| Purification | The antibody was purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide. |
| Concentration | 1 mg/ml |
| Specificity | ATR (Phospho-Ser428) antibody detects endogenous levels of ATR only when phosphorylated at Ser428. |
| Application Info | IHC: 1:50~1:100 ELISA: 1:40,000 |
| Gene Symbol | ATR |
|---|---|
| Gene Full Name | ATR serine/threonine kinase |
| Alias Symbols | ataxia telangiectasia and Rad3-related protein;FCTCS;FRAP-related protein 1;FRAP-related protein-1;FRP1;MEC1;MEC1, mitosis entry checkpoint 1, homolog;SCKL;SCKL1;serine/threonine-protein kinase ATR. |
| NCBI Gene Id | 545 |
| Protein Name | Serine/threonine-protein kinase ATR |
| Description of Target | Serine/threonine protein kinase which activates checkpoint signaling upon genotoxic stresses such as ionizing radiation (IR), ultraviolet light (UV), or DNA replication stalling, thereby acting as a DNA damage sensor. Recognizes the substrate consensus sequence [ST]-Q. Phosphorylates BRCA1, CHEK1, MCM2, RAD17, RPA2, SMC1 and p53/TP53, which collectively inhibit DNA replication and mitosis and promote DNA repair, recombination and apoptosis. Phosphorylates 'Ser-139' of histone variant H2AX/H2AFX at sites of DNA damage, thereby regulating DNA damage response mechanism. Required for FANCD2 ubiquitination. Critical for maintenance of fragile site stability and efficient regulation of centrosome duplication. |
| Uniprot ID | Q13535 |
| Protein Accession # | NP_001175.2 |
| Molecular Weight | 301 kDa |
- Protocol:
- Reconstitution & Storage Instructions
- Western Blotting/Immunoblotting (WB/IB) Protocol
- Immunohistochemistry (IHC) Protocol
- Immunocytochemistry (ICC) Protocol
- Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
- Blocking Peptide Competition Protocol (BPCP)
- Immunoprecipitation (IP) Protocol
- Antibody Array (AA) Protocol
- Tips Information:
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See our General FAQ page.
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What is the species homology for "ATR Antibody (Phospho-Ser428) (OAAF00519)"?
The tested species reactivity for this item is "". This antibody is predicted to have homology to "Human|Mouse|Rat".
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How long will it take to receive "ATR Antibody (Phospho-Ser428) (OAAF00519)"?
This item is available "Domestic: within 1-2 week delivery | International: 1-2 weeks".
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What buffer format is "ATR Antibody (Phospho-Ser428) (OAAF00519)" provided in?
This item is provided in "Liquid. PBS (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl.".
Additional format options may be available. For more information please contact info@avivasysbio.com. -
What are other names for "ATR Antibody (Phospho-Ser428) (OAAF00519)"?
This target may also be called "ataxia telangiectasia and Rad3-related protein;FCTCS;FRAP-related protein 1;FRAP-related protein-1;FRP1;MEC1;MEC1, mitosis entry checkpoint 1, homolog;SCKL;SCKL1;serine/threonine-protein kinase ATR." in publications.
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What is the shipping cost for "ATR Antibody (Phospho-Ser428) (OAAF00519)"?
The shipping cost for this item is $40 within the US. Please contact us for specific shipping prices for international orders.
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What is the guarantee for "ATR Antibody (Phospho-Ser428) (OAAF00519)"?
All Aviva products have been through rigorous validations and carry 100% satisfaction guarantee.
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Can I get bulk pricing for "ATR Antibody (Phospho-Ser428) (OAAF00519)"?
You can get bulk pricing for this item by going here.
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What is the molecular weight of the protein?
The molecular weight reported by Uniprot for this item is "301 kDa".
Please note observed molecular weights in western blot applications may differ depending on a variety of protein characteristics. -
What protocols are available for "ATR Antibody (Phospho-Ser428) (OAAF00519)"?
We may have detailed protocol data avaialble for this item. To learn more, please view the "Protocols & Data" tab on the product page.
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What are positive controls for "ATR"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What are negative controls for "ATR"?
We have listed RNA Seq and gene expression data in the "Target Info" tab. You may be able to find adequate positive controls there.
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What other proteins interact with "ATR"?
This protein has been reported to interact with "Protein Interactions". Please view the "Related Categories" tab on the product page for more information.
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What biological processes are associated with "ATR"?
This protein has been associated with "Biological Processes". Please view the "Related Categories" tab on the product page for more information.
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What cellular components are associated with "ATR"?
This protein has been associated with "Cellular Components". Please view the "Related Categories" tab on the product page for more information.
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What protein functions are associated with "ATR"?
This protein has been associated with "Protein Functions". Please view the "Related Categories" tab on the product page for more information.
