ATP6V1C1 antibody - N-terminal region (ARP58589_P050)

Catalog#: ARP58589_P050

• Size: 100 ul

• Gene Symbol: ATP6V1C1
• NCBI Gene Id: 528
• Official Gene Full Name: ATPase, H+ transporting, lysosomal 42kDa, V1 subunit C1
• Protein Name: V-type proton ATPase subunit C 1
• Swissprot Id: P21283
• Protein Accession #: NP_001686
• Nucleotide Accession #: NM_001695
• Alias Symbols: ATP6C, ATP6D, FLJ20057, VATC, Vma5
• Replacement Item: This antibody may replace item sc-36789 from Santa Cruz Biotechnology.
• Description of Target: ATP6V1C1 is a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene is one of two genes that encode the V1 domain C subunit proteins and is found ubiquitously. This C subunit is analogous but not homologous to gamma subunit of F-ATPases. Previously, this gene was designated ATP6D.This gene encodes a component of vacuolar ATPase (V-ATPase), a multisubunit enzyme that mediates acidification of intracellular compartments of eukaryotic cells. V-ATPase dependent acidification is necessary for such intracellular processes as protein sorting, zymogen activation, receptor-mediated endocytosis, and synaptic vesicle proton gradient generation. V-ATPase is composed of a cytosolic V1 domain and a transmembrane V0 domain. The V1 domain consists of three A and three B subunits, two G subunits plus the C, D, E, F, and H subunits. The V1 domain contains the ATP catalytic site. The V0 domain consists of five different subunits: a, c, c', c'', and d. Additional isoforms of many of the V1 and V0 subunit proteins are encoded by multiple genes or alternatively spliced transcript variants. This gene is one of two genes that encode the V1 domain C subunit proteins and is found ubiquitously. This C subunit is analogous but not homologous to gamma subunit of F-ATPases. Previously, this gene was designated ATP6D. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Entrez Gene record to access additional publications.
• Protein Size (# AA): 382
• Molecular Weight: 42kDa
• Host: Rabbit
• Clonality: Polyclonal
• Purification: Affinity Purified
• Application: WB
• RNA Seq: Find tissues and cell lines supported by RNA-seq analysis to express ATP6V1C1.
• Immunogen: The immunogen is a synthetic peptide directed towards the N terminal region of human ATP6V1C1
• Tested Species Reactivity: Human
• Predicted Homology Based on Immunogen Sequence: Cow: 100%; Dog: 100%; Guinea Pig: 93%; Horse: 100%; Human: 100%; Mouse: 100%; Rabbit: 100%; Rat: 100%; Zebrafish: 93%
• Peptide Sequence: Synthetic peptide located within the following region: ldafvegvvkkvaqymadvledskdkvqenllangvdlvtyitrfqwdma
• Product Format: Liquid. Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
• Reconstitution and Storage: For short term use, store at 2-8C up to 1 week. For long term storage, store at -20C in small aliquots to prevent freeze-thaw cycles.
• Concentration: Batch dependent within range: 100 ul at 0.5 - 1 mg/ml
• Protein Interactions: ATP6V1C1; IVNS1ABP; ATG7; DNM1L; TSN; STAT1; SNX2; SNX1; SHMT2; SHMT1; SARS; RDX; PAWR; NASP; IDE; GTF2A1; GSPT1; CTTN; PSMG3; ANP32E; TBC1D15; SNX6; VPS35; VPS29; LUC7L2; VTA1; ARFIP1; GSPT2; UBC; UBAC2; TXNDC17; MRPS35; CISD1; VASP; TYMS; SSR1; SRSF5; A
• Blocking Peptide: For anti-ATP6V1C1 (ARP58589_P050) antibody is Catalog # AAP58589 (Previous Catalog # AAPP35806)
• Subunit: C 1
• Target Reference: Rao,V.N., (2006) Nat. Cell Biol. 8 (2), 124-136
• Publications:

  Meo-Evoli, N; Almacellas, E; Massucci, FA; Gentilella, A; Ambrosio, S; Kozma, SC; Thomas, G; Tauler, A; V-ATPase: a master effector of E2F1-mediated lysosomal trafficking, mTORC1 activation and autophagy. 6, 28057-70 (2015). WB, Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat, Zebrafish 26356814

Protocol:

• Reconstitution Instructions
• Immunohistochemistry (IHC) Protocol
• Immunocytochemistry (ICC) Protocol
• Enzyme-Linked ImmunoSorbent Assay (ELISA) Protocol
• Western Blotting/Immunoblotting (WB/IB) Protocol
• Blocking Peptide Competition Protocol (BPCP)
• Immunoprecipitation (IP) Protocol

Tips Information:

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Product Protocols: ATP6V1C1 antibody tested with Human Hepg2 Cells (ARP58589_P050)