ATP6V1A ELISA Kit (Mouse) (OKEH03546)

Catalog No: OKEH03546

Size:96T

Mouse ATP6V1A ELISA kit standard curve

Product Info

• Predicted Species Reactivity: Mouse, Mus musculus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ATP6V1A ELISA Kit (Mouse) (OKEH03546) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for Atp6v1a has been pre-coated onto a 96-well plate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells and removed. A biotinylated detector antibody specific for Atp6v1a is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away. An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP generating a blue color product that changes yellow after adding acidic stop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample Atp6v1a captured in well.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 84%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 6.1% (n = 20)
  Mean Inter-assay CV%: <= 8.7% (n = 20)
• ELISA Kit Component:
  • Atp6v1a Microplate: 96 Wells (12 x 8 Well strips)
  • Atp6v1a Lyophilized Standard: 2
  • Sample Diluent: 1 x 20 mL
  • 100X Biotinylated Atp6v1a Detector Antibody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 10 mL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Urine, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.17 ng/mL
• Specificity: Natural and recombinant Mouse V-type proton ATPase catalytic subunit A
• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: Atp6v1a
• Gene Full Name: ATPase, H+ transporting, lysosomal V1 subunit A
• Alias Symbols: 70-kDa subunit;AI647066;Atp;Atp6a1;Atp6a2;Atp6v1a1;ATPase, H+ transporting, lysosomal (vacuolar proton pump), alpha 70 kDa;ATPase, H+ transporting, lysosomal 70kD, V1 subunit A;ATPase, H+ transporting, V1 subunit A;ATPase, H+ transporting, V1 subunit A1;lysosomal 70kDa;V;VA;VA68;vacuolar proton pump subunit alpha;V-ATPase 69 kDa subunit;V-ATPase subunit A;VPP2;V-type proton ATPase catalytic subunit A.
• NCBI Gene Id: 11964
• Protein Name: P50516
• Description of Target: V-type proton ATPase catalytic subunit A
• Uniprot ID: https://www.uniprot.org/uniprot/P50516
• Protein Accession #: NP_031534.2
• Nucleotide Accession #: NM_007508.5
• Protein Size (# AA): 617
• Molecular Weight: 68 kDa