ATP6AP2 ELISA Kit (Rat) (OKCD08928)

Catalog No: OKCD08928

Size:96WELLS

ATP6AP2 ELISA Kit (Rat) (OKCD08928) Standard Curve

Product Info

• Predicted Species Reactivity: Rat|Rattus norvegicus
• Application: Enzyme-linked Immunosorbent assay-Sandwich
• ELISA Kit Detection Method: Colorimetric
• ELISA Kit Duration: 3h
• ELISA Kit Principle: The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm +/- 10nm. The concentration of ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• ELISA Kit Range: 0.312-20ng/mL
• ELISA Kit Reproducibility: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) were tested 20 times on one plate, respectively. Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level ATPase, H+ Transporting, Lysosomal Accessory Protein 2 (ATP6AP2) were tested on 3 different plates, 8 replicates in each plate. CV(%) = SD/meanX100 Intra-Assay: CV<10% Inter-Assay: CV<12%
• ELISA Kit Component:
  • ATP6AP2 Microplate: 96 Wells (12 x 8 Well strips)
  • ATP6AP2 Lyophilized Standard: 2
  • 100X ATP6AP2 HRP-Detector Anitbody: 1 x 120 uL
  • 100X Avidin-HRP Conjugate: 1 x 120 uL
  • Standard Diluent: 1 x 20 mL
  • Detector Antibody Diluent: 1 x 12 mL
  • Conjugate Diluent: 1 x 12 mL
  • 30X Wash Buffer: 1 x 20 mL
  • TMB Substrate: 1 x 2 mL
  • Stop Solution: 1 x 20 mL
• Reconstitution and Storage: 2°C to 8°C|-20°C
• Sample Type: Tissue homogenates and other biological fluids.
• Sensitivity: < 0.128ng/mL

Target Info

• Gene Symbol: Atp6ap2
• Gene Full Name: ATPase H+ transporting accessory protein 2
• Alias Symbols: ATPase H(+)-transporting lysosomal accessory protein 2;ATPase H(+)-transporting lysosomal-interacting protein 2;ATPase, H(+)-transporting, lysosomal-interacting protein 2;ATPase, H+ transporting, lysosomal accessory protein 2;renin receptor;renin/prorenin receptor.
• NCBI Gene Id: 302526
• Protein Name: Renin receptor
• Description of Target: Functions as a renin and prorenin cellular receptor. May mediate renin-dependent cellular responses by activating ERK1 and ERK2. By increasing the catalytic efficiency of renin in AGT/angiotensinogen conversion to angiotensin I, it may also play a role in the renin-angiotensin system (RAS) (By similarity).
• Uniprot ID: Q6AXS4
• Protein Accession #: NP_001007092.1
• Nucleotide Accession #: NM_001007091.1