ATP6AP2 ELISA Kit (Human) (OKEH04445)

Catalog No: OKEH04445

Size:96 Wells

Human ATP6AP2 ELISA Kit standard curve

Product Info

• Predicted Species Reactivity: Human
• ELISA Kit Detection Method: Colorimetric, OD450 nm
• ELISA Kit Duration: ~ 3 Hours
• ELISA Kit Principle: Aviva Systems Biology ATP6AP2 ELISA Kit (Human) (OKEH04445) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATP6AP2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ATP6AP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATP6AP2 captured in the well.
• ELISA Kit Range: 0.312 - 20ng/mL
• ELISA Kit Recovery: Mean recovery when spiking into sample matrices at concentrations within the dynamic range: 110%
• ELISA Kit Reproducibility: Mean Intra-assay CV%: <= 5.8% (n = 20)
  Mean Inter-assay CV%: <= 8.7% (n = 20)
• ELISA Kit Component:
  • Anti-ATP6AP2 Microplate: 96 Wells (12 x 8 Well Strips)
  • ATP6AP2 Lyophilized Standard: 2 x 2 ng
  • Sample Diluent: 1 x 20 mL
  • ATP6AP2 Biotinylated Detector Antibody: 1 x 120 uL
  • Detector Antibody Diluent: 1 x 12 mL
  • HRP Avidin Conjugate: 1 x 120 uL
  • Conjugate Diluent: 1 x 12 mL
  • 25X Wash Buffer: 1 x 30 mL
  • TMB Substrate: 1 x 10 mL
  • Stop Solution: 1 x 10 mL
• Reconstitution and Storage: Store as indicated in product manual.
• Sample Type: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
• Sensitivity: 0.12 ng/mL
• Specificity: Natural and recombinant Human Renin receptor
• Publications:

  Plasma biomarkers for prediction of early tumor recurrence after resection of pancreatic ductal adenocarcinoma. Sci Rep. 11, 7499 (2021). 33820913

• Assay Info: Assay Methodology: Quantitative Sandwich ELISA

Target Info

• Gene Symbol: ATP6AP2
• Gene Full Name: ATPase H+ transporting accessory protein 2
• Alias Symbols: PRR, M8-9, MRXE, RENR, XMRE, XPDS, CDG2R, HT028, MRXSH, ELDF10, ATP6IP2, MSTP009, APT6M8-9, ATP6M8-9
• NCBI Gene Id: 10159
• Protein Name: Renin receptor
• Description of Target: This gene encodes a protein that is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases.
• Uniprot ID: O75787
• Protein Accession #: NP_005756.2
• Nucleotide Accession #: NM_005765.2
• Protein Size (# AA): 350
• Molecular Weight: 39 KDa