Catalog No: OKEH04445
Size:96 Wells
Price: $525.00
Availability: Domestic: within 2-3 weeks delivery | International: 2-3 weeks
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Datasheets/ManualsClick here to download product manual. As variation between lots may occur, always reference the lot-specific manual received with each kit.
Product Info
Predicted Species ReactivityHuman
ELISA Kit Detection MethodColorimetric, OD450 nm
ELISA Kit Duration~ 3 Hours
ELISA Kit PrincipleAviva Systems Biology ATP6AP2 ELISA Kit (Human) (OKEH04445) is based on standard sandwich enzyme-linked immuno-sorbent assay technology. An antibody specific for ATP6AP2 has been pre-coated onto a 96-wellplate (12 x 8 Well Strips) and blocked. Standards or test samples are added to the wells, incubated and removed. After washing, a biotinylated detector antibody specific for ATP6AP2 is added, incubated and followed by washing. Avidin-Peroxidase Conjugate is then added, incubated and unbound conjugate is washed away.An enzymatic reaction is produced through the addition of TMB substrate which is catalyzed by HRP to generating a blue color product that changes to yellow after adding acidicstop solution. The density of yellow coloration read by absorbance at 450 nm is quantitatively proportional to the amount of sample ATP6AP2 captured in the well.
ELISA Kit Range0.312 - 20ng/mL
ELISA Kit RecoveryMean recovery when spiking into sample matrices at concentrations within the dynamic range: 110%
ELISA Kit ReproducibilityMean Intra-assay CV%: <= 5.8% (n = 20)
Mean Inter-assay CV%: <= 8.7% (n = 20)
ELISA Kit Component
Anti-ATP6AP2 Microplate96 Wells (12 x 8 Well Strips)
ATP6AP2 Lyophilized Standard2 x 2 ng
Sample Diluent1 x 20 mL
ATP6AP2 Biotinylated Detector Antibody1 x 120 uL
Detector Antibody Diluent1 x 12 mL
HRP Avidin Conjugate1 x 120 uL
Conjugate Diluent1 x 12 mL
25X Wash Buffer1 x 30 mL
TMB Substrate1 x 10 mL
Stop Solution1 x 10 mL
Reconstitution and StorageStore as indicated in product manual.
Sample TypeSerum, Plasma, Tissue Homogenates, Cell Culture Supernates, Other Biological Fluids
Sensitivity0.12 ng/mL
SpecificityNatural and recombinant Human Renin receptor

Plasma biomarkers for prediction of early tumor recurrence after resection of pancreatic ductal adenocarcinoma. Sci Rep. 11, 7499 (2021). 33820913

Assay InfoAssay Methodology: Quantitative Sandwich ELISA
Gene SymbolATP6AP2
Gene Full NameATPase H+ transporting accessory protein 2
Alias SymbolsPRR, M8-9, MRXE, RENR, XMRE, XPDS, CDG2R, HT028, MRXSH, ELDF10, ATP6IP2, MSTP009, APT6M8-9, ATP6M8-9
NCBI Gene Id10159
Protein NameRenin receptor
Description of TargetThis gene encodes a protein that is associated with adenosine triphosphatases (ATPases). Proton-translocating ATPases have fundamental roles in energy conservation, secondary active transport, acidification of intracellular compartments, and cellular pH homeostasis. There are three classes of ATPases- F, P, and V. The vacuolar (V-type) ATPases have a transmembrane proton-conducting sector and an extramembrane catalytic sector. The encoded protein has been found associated with the transmembrane sector of the V-type ATPases.
Uniprot IDO75787
Protein Accession #NP_005756.2
Nucleotide Accession #NM_005765.2
Protein Size (# AA)350
Molecular Weight39 KDa
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